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    Transforming growth factor beta (TGF-β) isomers influence cell detachment of MG-63 bone cells

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    Publication date
    2015-12
    Author
    Sefat, Farshid
    Khaghani, Seyed A.
    Nejatian, T.
    Genedy, Mohamed A.
    Abdeldayem, Ali I.A.
    Moghaddam, Z.S.
    Denyer, Morgan C.T.
    Youseffi, Mansour
    Keyword
    MG63 Bone cell; TGF-β; Trypsinization; WSPR imaging
    Peer-Reviewed
    yes
    
    Metadata
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    Abstract
    Bone repair and wound healing are modulated by different stimuli. There is evidence that Transforming Growth Factor-beta (TGF-β) super-family of cytokines have significant effects on bone structure by regulating the replication and differentiation of chondrocytes, osteoblasts and osteoclasts. There is also significant evidence that interactions with extracellular matrix molecules influence cell behaviour. In this study cell surface attachment was examined via a trypsinization assay using various TGF-β isomers in which the time taken to trypsinize cells from the surface provided a means of assessing the strength of attachment. Three TGF-β isomers (TGF-β1, 2 and 3), four combined forms (TGF-β(1 + 2), TGF-β(1 + 3), TGF-β(2 + 3) and TGF-β(1 + 2 + 3)) along with four different controls (BSA, HCl, BSA/HCl and negative control) were investigated in this study. The results indicated that treatment with TGF-β1, 2, 3 and HCl decreased cell attachment, however, this effect was significantly greater in the case of TGF-β3 (p < 0.001) indicating perhaps that TGF-β3 does not act alone in cell detachment, but instead functions synergistically with signalling pathways that are dependent on the availability of hydrogen ions. Widefield Surface Plasmon Resonance (WSPR) microscope was also used to investigate cell surface interactions.
    URI
    http://hdl.handle.net/10454/8538
    Version
    No full-text available in the repository
    Citation
    Sefat F, Khaghani SA, Nejatian T, Genedy M, Abdeldayem A, Moghaddam ZS, Denyer, MCT and Youseffi, M (2015) Transforming growth factor beta (TGF-β) isomers influence cell detachment of MG-63 bone cells. Tissue and Cell, 47 (6): 567–574.
    Link to publisher’s version
    http://dx.doi.org/10.1016/j.tice.2015.08.002
    Type
    Article
    Collections
    Life Sciences Publications
    Engineering and Informatics Publications

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