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    Peptide-mediated growth and dispersion of Au nanoparticles in water via sequence engineering

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    Publication date
    2018-05
    Author
    Nguyen, M.A.
    Hughes, Zak E.
    Liu, Y.
    Li, Y.
    Swihart, M.T.
    Knecht, M.R.
    Walsh, T.R.
    Keyword
    Bio-nanotechnology
    Gold nanoparticles
    Peptides
    Molecular simulation
    Mutations
    Binding affinity
    Stabilization
    Rights
    © 2018 ACS. This document is the Accepted Manuscript version of a Published Work that appeared in final form in The Journal of Physical Chemistry C, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/acs.jpcc.8b02392
    Peer-Reviewed
    Yes
    
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    Abstract
    The use of peptides to nucleate, grow, and stabilize nanoparticles in aqueous media via non-covalent interactions offers new possibilities for creating functional, water-dispersed inorganic/organic hybrid materials, particularly for Au nanoparticles. Numerous previous studies have identified peptide sequences that both possess a strong binding affinity for Au surfaces and are capable of supporting nanoparticle growth in water. However, recent studies have shown that not all such peptide sequences can produce stable dispersions of these nanoparticles. Here, via integrated experiments and molecular modeling, we provide new insights into the many factors that influence Au nanoparticle growth and stabilization in aqueous media. We define colloidal stability by the absence of visible precipitation after at least 24 hours post-synthesis. We use binding affinity measurements, nanoparticle synthesis, characterization and stabilization assays, and molecular modeling, to investigate a set of sequences based on two known peptides with strong affinity for Au. This set of biomolecules is designed to probe specific sequence and context effects using both point mutations and global reorganization of the peptides. Our data confirm, for a broader range of sequences, that Au nanoparticle/peptide binding affinity alone is not predictive of peptide-mediated colloidal stability. By comparing nanoparticle stabilization assay outcomes with molecular simulations, we establish a correlation between the colloidal stability of the Au nanoparticles and the degree of conformational diversity in the surface-adsorbed peptides. Our findings suggest future routes to engineer peptide sequences for bio-based growth and dispersion of functional nanoparticles in aqueous media.
    URI
    http://hdl.handle.net/10454/15946
    Version
    Accepted Manuscript
    Citation
    Nguyen MA, Hughes ZE, Liu Y et al (2018) Peptide-mediated growth and dispersion of Au nanoparticles in water via sequence engineering. The Journal of Physical Chemistry C. 122(21): 11532-11542.
    Link to publisher’s version
    http://dx.doi.org/10.1021/acs.jpcc.8b02392
    Type
    Article
    Collections
    Life Sciences Publications

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