Alterations in intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) in human endothelial cells

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dc.contributor.authorHabas, Khaled S.A.*
dc.contributor.authorShang, Lijun*
dc.date.accepted11/09/2018
dc.date.accessioned2018-09-28T11:54:15Z
dc.date.application12/09/2018
dc.date.available2018-09-28T11:54:15Z
dc.date.issued2018-10
dc.descriptionYes
dc.description.abstractAlterations of Endothelial cells (ECs) play a critical role in different pathogenesis of many serious human diseases, and dysfunction of the vascular endothelium is an indicator for human disorders. Endothelial dysfunction is considered to be an early indicator for atherosclerosis, which is characterised by overexpression of adhesion molecules, including intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1). Hydrogen peroxide (H2O2) released via neutrophils is an important mediator of endothelial cell function. Ambient production of superoxide anion (O2−) and subsequently H2O2 at low levels is critical for regulating endothelial cell functions and proliferation. In this study, we investigated the effects of H2O2 on the expression of adhesion molecules VCAM-1 and ICAM-1 in cultured human umbilical vein endothelial cells (HUVECs). Intracellular superoxide anion production was detected by using p-Nitro Blue Tetrazolium (NBT) assay. Our results showed that administration of 100μM of H2O2 on HUVECs for 2, 6, 12 and 24 h induced a time-dependent increase in ICAM-1 and VCAM-1 mRNA and protein expression levels with a significant increase observed from 6 h. HUVECs exposed to H2O2 exhibit increased O2−, suggesting that H2O2 induced oxidative stress may be a reasonable for atherosclerosis. This increase can be reduced by the flavonoid, N-acetyl cysteine (NAC). The modulation of endothelial cell function through this mechanism may underlie the contribution of H2O2 to the development of vascular disease.
dc.identifier.citationHabas K and Shang L (2018) Alterations in intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) in human endothelial cells. Tissue and Cell. 54: 139-143.
dc.identifier.doihttps://doi.org/10.1016/j.tice.2018.09.002
dc.identifier.urihttp://hdl.handle.net/10454/16587
dc.language.isoen
dc.openaccess.statusopenAccess
dc.rightsCrown Copyright © 2018 Published by Elsevier Ltd. All rights reserved. Reproduced in accordance with the publisher's self-archiving policy. This manuscript version is made available under the CC-BY-NC-ND 4.0 license.
dc.rights.licenseCC-BY-NC-ND
dc.status.refereedYes
dc.subjectHuman umbilical vein endothelial cells (HUVECs)
dc.subjectIntercellular adhesion molecule 1(CAM-1)
dc.subjectVascular cell adhesion molecule 1 (VCAM-1)
dc.subjectHydrogen peroxide (H2O2)
dc.titleAlterations in intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) in human endothelial cells
dc.typeArticle
dc.type.versionAccepted manuscript
dspace.entity.typePublication
refterms.dateFOA2018-09-28T11:54:19Z
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