Tracking cell proliferation using a nanotechnology based approach

To develop an efficient nanotechnology fluorescence-based method to track cell proliferation to avoid the limitations of current cell-labeling dyes. Material & methods: Synthesis, PEGylation, bifunctionalization and labeling with a fluorophore (Cy5) of 200 nm polystyrene nanoparticles (NPs) were performed. These NPs were characterized and assessed for in vitro long-term monitoring of cell proliferation. Results: The optimization and validation of this method to track long-term cell proliferation assays have been achieved with high reproducibility, without cell cycle disruption. This method has been successfully applied in several adherent and suspension cells including hard-to-transfect cells and isolated human primary lymphocytes. Conclusion: A novel approach to track efficiently cellular proliferation by flow cytometry using fluorescence labeled NPs has been successfully developed.

URI

http://hdl.handle.net/10454/13342

Version

Accepted manuscript

Citation

Altea-Manzano P, Unciti-Broceta JD, Cano-Cortes V, Ruiz-Blas MP, Valero-Grinan Teresa M, Diaz-Mochon JJ and Sanchez-Martin R (2017) Tracking cell proliferation using a nanotechnology-based approach. Nanomedicine. 12(13): 1591-1605.

Link to Version of Record

https://doi.org/10.2217/nnm-2017-0118

Type

Article