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Tracking cell proliferation using a nanotechnology based approach
Altea-Manzano, P. Unciti-Broceta, J.D. Cano-Cortes, V. Ruiz-Blas, M.P. Valero-Grinan, Teresa M. Diaz-Mochon, J.J. Sanchez-Martin, R.
Altea-Manzano, P.
Unciti-Broceta, J.D.
Cano-Cortes, V.
Ruiz-Blas, M.P.
Valero-Grinan, Teresa M.
Diaz-Mochon, J.J.
Sanchez-Martin, R.
Publication Date
2017-07
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© 2017 IOP Publishing. Reproduced in accordance with the publisher's self-archiving policy.
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Abstract
To develop an efficient nanotechnology fluorescence-based method to track cell proliferation to avoid the limitations of current cell-labeling dyes. Material & methods: Synthesis, PEGylation, bifunctionalization and labeling with a fluorophore (Cy5) of 200 nm polystyrene nanoparticles (NPs) were performed. These NPs were characterized and assessed for in vitro long-term monitoring of cell proliferation. Results: The optimization and validation of this method to track long-term cell proliferation assays have been achieved with high reproducibility, without cell cycle disruption. This method has been successfully applied in several adherent and suspension cells including hard-to-transfect cells and isolated human primary lymphocytes. Conclusion: A novel approach to track efficiently cellular proliferation by flow cytometry using fluorescence labeled NPs has been successfully developed.
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Accepted manuscript
Citation
Altea-Manzano P, Unciti-Broceta JD, Cano-Cortes V, Ruiz-Blas MP, Valero-Grinan Teresa M, Diaz-Mochon JJ and Sanchez-Martin R (2017) Tracking cell proliferation using a nanotechnology-based approach. Nanomedicine. 12(13): 1591-1605.
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