The commonly-used DNA probe for diffusely-adherent Escherichia coli cross-reacts with a subset of enteroaggregative E. coli

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dc.contributor.authorSnelling, Anna M.
dc.contributor.authorMacfarlane-Smith, Louissa
dc.contributor.authorFletcher, Jonathan
dc.contributor.authorOkeke, Iruka N.
dc.date.accessioned2025-07-30T15:09:15Z
dc.date.available2011-02-07T14:50:30Z
dc.date.available2025-07-30T15:09:15Z
dc.date.issued21/12/2009
dc.descriptionYes
dc.description.abstractBackground. The roles of diffusely-adherent Escherichia coli (DAEC) and enteroaggregative E. coli (EAEC) in disease are not well understood, in part because of the limitations of diagnostic tests for each of these categories of diarrhoea-causing E. coli. A HEp-2 adherence assay is the Gold Standard for detecting both EAEC and DAEC but DNA probes with limited sensitivity are also employed. Results. We demonstrate that the daaC probe, conventionally used to detect DAEC, cross-reacts with a subset of strains belonging to the EAEC category. The cross hybridization is due to 84% identity, at the nucleotide level, between the daaC locus and the aggregative adherence fimbriae II cluster gene, aafC, present in some EAEC strains. Because aaf-positive EAEC show a better association with diarrhoea than other EAEC, this specific cross-hybridization may have contributed to an over-estimation of the association of daaC with disease in some studies. We have developed a discriminatory PCR-RFLP protocol to delineate EAEC strains detected by the daaC probe in molecular epidemiological studies. Conclusions. A PCR-RFLP protocol described herein can be used to identify aaf-positive EAEC and daaC-positive DAEC and to delineate these two types of diarrhoeagenic E. coli, which both react with the daaC probe. This should help to improve current understanding and future investigations of DAEC and EAEC epidemiology.
dc.identifier.citationSnelling AM, Macfarlane-Smith LR, Fletcher JN et al (2009) The commonly-used DNA probe for diffusely-adherent Escherichia coli cross-reacts with a subset of enteroaggregative E. coli. BMC Microbiology. 9: 269.
dc.identifier.doihttps://doi.org/10.1186/1471-2180-9-269
dc.identifier.urihttps://bradscholars.brad.ac.uk/handle/10454/4778.2
dc.language.isoen
dc.openaccess.statusopenAccess
dc.rights© 2009 Snelling et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.rights.licenseCC-BY
dc.status.refereedYes
dc.subjectDiffusely-adherent Escherichia coli (DAEC)
dc.subjectEnteroaggregative E. coli (EAEC)
dc.subjectDiarrhoea
dc.subjectDNA probes
dc.subjectA HEp-2 adherence assay
dc.subjectDaaC probe
dc.subjectPCR-RFLP
dc.titleThe commonly-used DNA probe for diffusely-adherent Escherichia coli cross-reacts with a subset of enteroaggregative E. coli
dc.typeArticle
dc.type.versionPublished version
dspace.entity.typePublication
refterms.dateFOA2018-07-19T04:27:11Z
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