Antibiotic functionalised polymers reduce bacterial biofilm and bioburden in a simulated infection of the cornea

View/ Open
Swift_Biomaterials_Science_Final.pdf (1.565Mb)
Download
Publication date
2018Author
Doroshenko, N.Rimmer, Stephen
Hoskins, Richard
Garg, P.
Swift, Thomas

Spencer, Hannah L.M.
Lord, Rianne M.
Katsikogianni, Maria

Pownall, D.
MacNeil, S.
Douglas, C.W.I.
Shepherd, J.
Rights
© The Royal Society of Chemistry 2018. Open Access article. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.Peer-Reviewed
YesOpen Access status
openAccessAccepted for publication
17/05/2018
Metadata
Show full item recordAbstract
Microbial keratitis can arise from penetrating injuries to the cornea. Corneal trauma promotes bacterial attachment and biofilm growth, which decrease the effectiveness of antimicrobials against microbial keratitis. Improved therapeutic efficacy can be achieved by reducing microbial burden prior to antimicrobial therapy. This paper assesses a highly-branched poly(N-isopropyl acrylamide) with vancomycin end groups (HB-PNIPAM-van), for reducing bacterial attachment and biofilm formation. The polymer lacked antimicrobial activity against Staphylococcus aureus, but significantly inhibited biofilm formation (p = 0.0008) on plastic. Furthermore, pre-incubation of S. aureus cells with HB-PNIPAM-van reduced cell attachment by 50% and application of HB-PNIPAM-van to infected ex vivo rabbit corneas caused a 1-log reduction in bacterial recovery, compared to controls (p = 0.002). In conclusion, HB-PNIPAM-van may be a useful adjunct to antimicrobial therapy in the treatment of corneal infections.Version
Published versionCitation
Doroshenko N, Rimmer S, Hoskins R et al (2018) Antibiotic functionalised polymers reduce bacterial biofilm and bioburden in a simulated infection of the cornea. Biomaterials Science. 6(8): 2101-2109.Link to Version of Record
https://doi.org/10.1039/C8BM00201KType
Articleae974a485f413a2113503eed53cd6c53
https://doi.org/10.1039/C8BM00201K