Calculation of electrophoretic mobility in mixed solvent buffers in capillary zone electrophoresis using a mixture response surface method.
Jouyban, A. Grosse, S.C. Coleman, M.W. Chan, H.K. Kenndler, E. Clark, Brian J.
Jouyban, A.
Grosse, S.C.
Coleman, M.W.
Chan, H.K.
Kenndler, E.
Clark, Brian J.
Publication Date
2009-10-27
End of Embargo
Supervisor
Rights
Peer-Reviewed
Yes
Open Access status
closedAccess
Accepted for publication
Institution
Department
Awarded
Embargo end date
Collections
Additional title
Abstract
The electrophoretic mobilities of three beta-blocker drug practolol, timolol and propranolol, have been measured in electrolyte systems with mixed binary and ternary water-methanol-ethanol solvents with acetic acid/sodium acetate as buffer using capillary electrophoresis. The highest mobilities for the analytes studied have been observed in pure aqueous. the lowest values in ethanolic buffers The measured electrophoretic mobilities have been used to evaluate the accuracy of a mathematical model based on a mixture response surface method that expresses the mobility as a function of the solvent composition. Mean percentage error (MPE) has been computed considering experimental and calculated mobilities as an accuracy criterion. The obtained MPE for practolol, timolol and propranolol in the binary mixtures are between 0.9 and 2.6%, in the ternary water-methanol-ethanol solvent system the MPE was about 2.7%. The MPE values resulting from the proposed equation lie within the experimental relative standard deviation values and can he considered as an acceptable error.
Version
No full-text in the repository
Citation
Jouyban, A., Grosse, S.C., Clark, B.J. and Chan, H.K. et al. (2002). Calculation of electrophoretic mobility in mixed solvent buffers in capillary zone electrophoresis using a mixture response surface method. The Analyst. Vol. 127, No. 9, pp. 1188-1192.
Link to publisher’s version
Link to published version
Link to Version of Record
Type
Article