Ardila-Fierro, K.Crawford, Deborah E.Körner, A.James, S.L.Bolm, C.Hernández, J.G.2020-03-032020-03-092020-03-032020-03-092018-01Ardila-Fierro KJ, Crawford DE, Körner A et al (2018) Papain-catalysed mechanochemical synthesis of ogliopeptides by milling and twin-screw extrusion: application in the Juliá–Colonna enantioselective epoxidation. Green Chemistry. 20(6): 1262-1269.RMSID:212563912http://hdl.handle.net/10454/17696NoThe oligomerisation of L-amino acids by papain was studied in a mixer ball mill and in a planetary ball mill. The biocatalyst proved stable under the ball milling conditions providing the corresponding oligopeptides in good to excellent yields and with a variable degree of polymerisation. Both parameters were found to be dependent on the reaction conditions and on the nature of the amino acid (specifically on its side-chain size and hydrophobicity). In addition, the chemoenzymatic oligomerisation was demonstrated by utilising twin-screw extrusion technology, which allowed for a scalable continuous process. Finally, the synthesised oligo(L-Leu) 2b proved to be active as a catalyst in the Juliá–Colonna enantioselective epoxidation of chalcone derivatives.enOligomerisationPapainBall millingTwin-screw extrusionJuliá–Colonna enantioselective epoxidationArticlehttps://doi.org/10.1039/C7GC03205F2020-03-03