Graham, Anne MNaseem, Khalid M.Oberprieler, Nikolaus G.Riba, RocioRoberts, WayneHomer-Vanniasinkam, Shervanthi2009-08-172009-08-172007Oberprieler, N.G., Roberts, W., Riba, R., Graham, A.M., Naseem, K.M. and Homer-Vanniasinkam, S. (2007). cGMP-independent inhibition of integrin alphaIIbbeta3- mediated platelet adhesion and outside-in signalling by nitric oxide. FEBS Letters. Vol. 581, No. 7, pp. 1529-34.http://hdl.handle.net/10454/3313NoWe examined the influence of S-nitrosoglutathione (GSNO) on alpha(IIb)beta(3) integrin-mediated platelet adhesion to immobilised fibrinogen. GSNO induced a time- and concentration-dependent inhibition of platelet adhesion. Inhibition was cGMP-independent and associated with both reduced platelet spreading and protein tyrosine phosphorylation. To investigate the cGMP-independent effects of NO we evaluated integrin beta(3) phosphorylation. Adhesion to fibrinogen induced rapid phosphorylation of beta(3) on tyrosines 773 and 785, which was reduced by GSNO in a cGMP independent manner. Similar results were observed in suspended platelets indicating that NO-induced effects were independent of spreading-induced signalling. This is the first demonstration that NO directly regulates integrin beta(3) phosphorylation.enNitric oxidecGMPPlateletsIntegrin ¿IIbß3Tyrosine phosphorylationArticlehttps://doi.org/10.1016/j.febslet.2007.02.072