Djidja, M-C.Francese, S.Claude, E.Loadman, PaulSutton, Chris W.Shnyder, StevenCooper, Patricia A.Patterson, Laurence H.Carolan, V.A.Clench, M.R.2016-10-062016-10-0601/04/2013Djidja M, Francese S, Claude E et al (2013) Targeting of Hypoxia in AQ4N-treated Tumour Xenografts by MALDIIon Mobility Separation-Mass Spectrometry Imaging. Current Analytical Chemistry. 9(2): 212-225.http://hdl.handle.net/10454/9533NoHypoxia is a common feature observed in solid tumours. It is a target of interest in oncology as it has been found to be closely associated with tumour progression, metastasis and aggressiveness and confers resistance to a variety of chemotherapeutic agents as well as radiotherapy. AQ4N, also known as banoxatrone or 1,4-bis-[2-(dimethylamino-Noxide) ethyl]amino-5,8-dihydroxyanthracene-9,10-dione is a very promising bioreductive prodrug. This paper, describes an application of MALDI-MSI combined with ion mobility separation and an "on-tissue" bottom up proteomic strategy to obtain proteomic data from AQ4N dosed tumour xenograft tissue sections. These data are then correlated with the drug distribution determined also using MALDI-ion mobility separation-mass spectrometry imaging (MALDI-IMS-MSI). PCA-DA and OPLS-DA have been used to compare treated and untreated xenografts and of note is the marked increase in expression of Histone H3.enHypoxiaMALDIIon mobilityMass spectrometry imagingAQ4NArticlehttps://doi.org/10.2174/1573411011309020007