Antimicrobial resistance (AMR) is one of the most alarming global public health challenges of the 21st century. Over 3 million antimicrobial-resistant infections occur in the United States annually, with nearly 50,000 cases being fatal. Innovations in drug discovery methods and platforms are crucial to identify novel antibiotics to combat AMR. We present the isolation and characterization of potentially novel antibiotic lead compounds produced by the cross-feeding of two rhizosphere bacteria, Acinetobacter sp. RIT 592 and Exiguobacterium sp. RIT 594. We used solid-phase extraction (SPE) followed by liquid chromatography (LC) to enrich antibiotic extracts and subsequently mass spectrometry (MS) analysis of collected fractions for compound structure identification and characterization. The MS data were processed through the Global Natural Product Social Molecular Networking (GNPS) database. The supernatant from RIT 592 induced RIT 594 to produce a cocktail of antimicrobial compounds active against Gram-positive and negative bacteria. The GNPS analysis indicated compounds with known antimicrobial activity in the bioactive samples, including oligopeptides and their derivatives. This work emphasizes the utility of microbial community-based platforms to discover novel clinically relevant secondary metabolites. Future work includes further structural characterization and antibiotic activity evaluation of the individual compounds against pathogenic multidrug-resistant (MDR) bacteria.

Objective This project reconstructs the early life history of the child in industrial London using incremental dentine analysis of both adults (survivors) and children (non-survivors) within a single 19th century London burial population to investigate the effects which changing social and economic conditions during Industrialisation had on their diet, health, and quality of life. Materials & Methods Macroscopic analysis was performed on the excavated assemblage (n=514) of New Bunhill Fields burial ground, Southwark, London (1821-53) to identify those with skeletal evidence of childhood stress (LEH, rickets, residual rickets) who were suitable for isotopic analysis. Radiographic analysis of the dental pulp chamber was also performed on these individuals to identify childhood vitamin D deficiency. A total of 20 adults (20/157) and 22 non-adults (22/357) were chosen for carbon (δ13C) and nitrogen (δ15N) stable isotope analysis. Incremental dentine analysis was performed on the first permanent molar and isotopic profiles were created spanning from birth until tooth completion/death during tooth development. Results The isotopic data indicated that adult and non-adult diet was consistent with that observed in other 19th century London burial populations. Evidence for breastfeeding was widespread amongst survivors and non-survivors. Radiographic results indicated that vitamin D deficiency during early childhood was much more prevalent within the burial group than skeletal evidence suggested, and it was observed more frequently in those who survived into adulthood (80%) than in those who died during childhood (42%). Concurrent evidence of isotopic physiological stress with LEH formation was observed in some but not all individuals. A pattern of opposing co-variance was observed often in early infancy, most frequently in non-survivors, and isotopic evidence for physiological stress was also observed in the final dentine increments of some who died during tooth development. There was a tentative association observed between adult age-at-death and skeletal stress indicators in early childhood, where the average age-at-death was older in those with fewer childhood stress indicators. Conclusions & Significance This research project found that early life stress was common within this urban community and was associated with negative outcomes later in life, especially amongst those who died in childhood.

"Fixing Illicit Financial Flows between Nigeria, the UK, and UAE: Corruption's Bermuda Triangle" delves into one of the most pressing challenges in global finance - illicit financial flows (IFFs). This groundbreaking book examines how vast sums of Nigeria’s wealth vanish into the financial systems of the UK and UAE, depleting the country's economic resources. With a focus on real estate, banking, and professional sectors, the authors critically assess the existing laws and regulations in all three countries and explore the gaps that allow corruption and money laundering to thrive. Supported by empirical research, the book highlights the urgent need for reform in international financial practices and offers innovative solutions to curb the outflow of illicit wealth from Nigeria. A must-read for policymakers, legal scholars, and anyone interested in the intersection of law, finance, and development in Africa.

In this paper, we pay tribute to the career-long interest of Prof Peter Akkermans in the mortuary practices of prehistoric southwest Asia. It highlights the significant discovery of burial grounds at Tell Sabi Abyad and the diverse range of mortuary behaviours observed at this key Late Neolithic site. While traditional approaches to the treatment of the dead often focus on social status, this paper proposes a fresh perspective by examining the agency of the bereaved and the impact of their emotional needs on mortuary rituals. Drawing on an extensive dataset of over 300 burials, the paper centres on a subset of burials from Tell Sabi Abyad I and III, dating back to the 7th and early 6th millennium BCE. In contrast to the typical interments, these burials reveal extended interaction with the deceased, involving bodily manipulation and close spatial proximity to the living. In this paper, we delve into new interpretations of this evidence, shedding light on the emotional relationships between the living and the dead. We explore possible motivations behind maintaining a tangible connection with the deceased and examine whether such practices offer insights into experiences of grief and bereavement – dimensions often overlooked in archaeological analysis. By incorporating emotion, sensory experiences, and mourning into the interpretation of mortuary evidence, in this paper, we aim to enrich our understanding of mortuary practices’ significance in southwest Asia’s prehistoric context.

To investigate the shear performance of poplar laminated veneer lumber (LVL) beams with holes in bending-shear spans, six specimens were designed and tested by four-point bending tests. Among these, five specimens were provided with a single hole of varying diameter-to-height ratio in the bending-shear span and two of these beams were also reinforced with circumferential carbon fiber reinforced polymer (CFRP) wrap layers. Furthermore, a 3D finite element models for poplar LVL beams with a hole were established, based on the extended finite element method (XFEM) using ABAQUS software. The validated model was utilized to conduct parametric studies on the diameter-to-height ratio, the hole shape, and the vertical eccentricity ratio. A simplified theoretical analysis for predicting the cracking and ultimate loads for LVL beam with a hole was also proposed. The results indicated that beams without a hole failed due to bending, characterized by mid-span tension cracks, whereas beams with a hole exhibited shear failure along the beam's grain direction due to stress concentration around the holes. The maximum normal tensile strain perpendicular to grain around the hole had an angle of 45° or 225° relative to the beam's longitudinal axis, consistent with the crack initiation angle. As the diameter-to-height ratio increased, the cracking and ultimate loads of beams with a hole decreased, indicating more brittle failure characteristics. The circular hole beam showed significant improvements in cracking and ultimate loads compared with the square hole beam with side length equal to the diameter of the circular hole. When the hole center's vertical eccentricity was in the compression zone, an increase in vertical eccentricity led to enhancements in both the cracking load and ultimate loads. Wrapping the beam with CFRP sheet around the hole effectively mitigated crack propagation, enhancing the load-bearing capacity of beams. The simplified formulas provided accurate prediction for the ultimate load, but highly overestimated the cracking and ultimate loads for poplar LVL beams with a hole. The research findings can be provided as a technical support for the design and application of LVL beams with holes.

This paper summarizes the results of a recent geophysical investigation carried out at Porolissum, which is considered to be one of the most significant Roman sites in Romania. The geophysical survey was carried out within the latus dextrum of the fort, which is the same location that had been the subject of earlier geophysical surveys as well as older archaeological excavations (1970s) that had uncovered a multiroom building. A cesium vapor total field magnetometer and a multi-electrode resistivity meter for a dense Electrical Resistivity Tomography (ERT) survey were used. Eighty parallel ERT profiles in combination with the emerging total field magnetic data and an antecedent magnetic survey allowed us to complete a more precise interpretation regarding the building that once existed on the left side of Porolissum’s Principia (the commander’s house). In contrast to the magnetic survey, which only reveals a part of the building’s architecture, the ERT survey provides a comprehensive view of the structure’s layout. More than 20 rooms could be positively identified, and the existence of further rooms might be deduced from the data. The ERT scan revealed the existence of the building’s northern external wall as well, which is not reflected on the magnetic map. Because some parts of the building are not visible on the magnetic map, we can assume that the building was constructed with at least two types of rocks (magmatic and sedimentary). In addition to the archaeological interpretation of the geophysical anomalies, a number of discussions concerning the connection between our survey and the geology of the area were held. The complementarity of the magnetic and resistivity results prompted us to conceive a preliminary 3D reconstruction of the building. Even if the building function is unknown in the absence of reliable archaeological data, it could have been a storage building, a second praetorium, a valetudinarium (hospital), or an armamentarium (weapons storage building). The illustrative reconstruction was completed taking into consideration that the building was a Roman military hospital, which, based on the available data, may be considered a credible assumption.

The sustained and controlled delivery of antimicrobial drugs has been largely studied using nanomaterials, like metal organic frameworks (MOFs), and various polymers. However, not much attention has been given to combining MOFs and biodegradable polymers towards the potentially more sustained release of active pharmaceutical ingredients. Herein, we report a comparative study of two widely used antimicrobial drugs, cephalexin and metronidazole, from zinc-based MOF-5 incorporated into biodegradable polycaprolactone (PCL) and poly-lactic glycolic acid (PLGA) composites. Cephalexin and metronidazole were separately loaded into MOF-5 post-synthetically, followed by their integration into biodegradable PLGA and PCL composites. The pristine MOF-5 and the loaded MOFs were thoroughly characterised using Fourier-transformed infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), thermogravimetric analysis (TGA) and powder X-ray diffraction (PXRD). Ultraviolet-visible (UV-Vis) spectroscopy studies were carried out to assess the release of the drugs in PBS for up to 72 h, showing a cumulative release of 24.95 wt% and 27.84 wt% for cephalexin and metronidazole, respectively. The antibacterial properties of the pristine MOF, pure drugs, drug-loaded MOFs and the loaded composites were assessed against Gram-positive and Gram-negative bacterial strains, Staphylococcus aureus or Staphylococcus epidermidis and Escherichia coli or Acinetobacter baumanii, respectively. A cephalexin-loaded MOF-5 composite of PCL (PCL-ceph@MOF-5) showed the best efficiency for the controlled release of drugs to inhibit the growth of the bacteria compared to the other composites. This study demonstrates that the combination of MOFs with biodegradable polymers can provide an efficient platform for the sustained release of antimicrobial drugs and can be a promising tool to manage antimicrobial resistance (AMR).

As universities extend the scope of their tasks as educational institutions to include technology transfer and commercialization of their faculty’s and students’ research outputs, it becomes necessary to investigate internal systems that facilitate such processes. Existing literature on technology transfer in the Philippines is mostly focused on the transfer of a specific technology, industry–academe research and development (R&D) collaboration, the experience in a specific industry, or the experience in public Philippine higher education institutions (HEIs). The perspectives of educators and researchers who are the recipients of technology transfer services within Philippine HEIs have, so far, not yet been investigated. This paper aims to fill that gap by interviewing eight educators from four colleges in a Philippine HEI. The findings primarily reveal the importance of internal communications in the university technology transfer process. They also reveal that, aside from entrepreneurship training, educators need to understand the intellectual property rights system to see the potential benefits of intellectual property protection and be able to relate intellectual property rights to their research work. This paper therefore recommends that, to promote intellectual property awareness and academic entrepreneurship in the country, HEIs need to allocate resources for intellectual property education and entrepreneurship training to encourage active participation of researchers in Philippine HEIs in the formal technology transfer process.

Purpose. To investigate the subjective refraction techniques of UK optometrists and their influence on patient-reported problems with new spectacles. Methods. First, an investigation from optical practices’ perspective, with a study investigating the frequency and causes of patient complaints. Three questionnaires follow; i) quantification of patient-reported symptoms with new spectacles, ii) the methods of refraction used by clinicians and iii) part-refracting as a special case of part-prescribing. Finally, the typical cylinder changes prescribed in patients’ refractive history are examined. Results. 2.3% of eye examinations resulted in rechecks. Cylinders were implicated in 38% of causes, of which 42% were oblique. 83% of rechecks were due to inaccurate measurement of prescription; presenting symptoms, prescription changes and improvements in visual acuity (VA) were often not reconciled and 93% reported not measuring VA to full threshold. The change in ocular astigmatism from with- to against-the-rule with age was more than three times more likely to pass through oblique axes than a spherical prescription. 36% of eyes were found to have an oblique cylinder prescribed at least once and of these, 78% were transitory in nature. Conclusions. Subjective refraction, visual acuity measurement, analysis of refractive change and prescribing techniques were often poor and cylinder changes, particularly oblique, were identified as a cause of increasing rechecks with patient age. These are fundamental aspects of optometry, yet need to be more prominent in continuing professional development.

Natural Language Processing (NLP) technology has the potential to provide a non-invasive, cost-effective method using a timely intervention for detecting early-stage language and cognitive decline in individuals concerned about their memory. The proposed pre-screening language and cognition assessment model (PST-LCAM) is based on the functional linguistic model Role and Reference Grammar (RRG) to analyse and represent the structure and meaning of utterances, via a set of language production and cognition parameters. The model is trained on a DementiaBank dataset with markers of cognitive decline aligned to the global deterioration scale (GDS). A hybrid approach of qualitative linguistic analysis and assessment is applied, which includes the mapping of participants´ tasks of speech utterances and words to RRG phenomena. It uses a metric-based scoring with resulting quantitative scores and qualitative indicators as pre-screening results. This model is to be deployed in a user-centred conversational assessment platform.