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dc.contributor.authorWedley, Amy L.*
dc.contributor.authorElajnef, Hasan M.*
dc.contributor.authorFletcher, Jonathan N.*
dc.date.accessioned2016-10-19T16:08:12Z
dc.date.available2016-10-19T16:08:12Z
dc.date.issued2013
dc.identifier.citationWedley AL, Elajnef HM and Fletcher JN (2013) Characterization of a novel EAST-negative enteropathogenic E.coli strain implicated in a food-borne outbreak of diarrhoea in adults. APMIS 121(6): 494-502.en_US
dc.identifier.urihttp://hdl.handle.net/10454/9985
dc.descriptionYesen_US
dc.description.abstractEnteropathogenic Escherichia coli (EPEC) is usually associated with outbreaks and sporadic cases of severe infantile diarrhoea in the developing world, and less commonly with sporadic cases in developed countries. Very little evidence indicates that EPEC is a food-borne pathogen for adults. In a previous study, two groups of adult travellers became ill, and eae+ E. coli of serogroup O111 was isolated from affected individuals and epidemiologically linked to food consumption. Here the strain responsible was further investigated and characterized as an unusual atypical EPEC. PCR analysis of the designated type isolate showed the presence of the rorf1 and espB genes of the LEE pathogenicity island, which was inserted at the chromosomal selC locus. The isolate was negative for the enteroaggregative E. coli EAST-1 toxin present in other strains of EPEC associated with food-borne outbreaks. The strain adhered sparsely to HEp-2 cell monolayers in a diffuse manner, but fluorescent actin staining demonstrated that it was capable of inducing polymerization of actin at the sites of bacterial attachment. Strain P2583 is the first EAST-negative EPEC to be confirmed as a cause of outbreaks of infection in adults following the consumption of contaminated food or water.en_US
dc.language.isoenen_US
dc.rights© 2013 Wiley. This is the peer reviewed version of the following article: Wedley AL, Elajnef HM and Fletcher JN (2013) Characterization of a novel EAST-negative enteropathogenic E.coli strain implicated in a food-borne outbreak of diarrhoea in adults. APMIS 121(6): 494-502., which has been published in final form at http://dx.doi.org/10.1111/apm.12024. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.en_US
dc.subjectEnteropathogenic Escherichia coli (EPEC); Diarrhoea; Food-borne outbreaks of diarrhoea; EAST-negative EPECen_US
dc.titleCharacterization of a novel EAST-negative enteropathogenic E. coli strain implicated in a food-borne outbreak of diarrhoea in adultsen_US
dc.status.refereedYesen_US
dc.date.Accepted2012-10-04
dc.date.application2012-11-08
dc.typeArticleen_US
dc.type.versionAccepted Manuscripten_US
dc.identifier.doihttps://doi.org/10.1111/apm.12024
refterms.dateFOA2018-07-27T01:39:15Z


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