Show simple item record

dc.contributor.authorShaheed, Sadr-ul*
dc.contributor.authorRustogi, Nitin*
dc.contributor.authorScally, Andy J.*
dc.contributor.authorWilson, J.*
dc.contributor.authorThygesen, H.*
dc.contributor.authorLoizidou, M.A.*
dc.contributor.authorHadjisavvas, A.*
dc.contributor.authorHanby, A.*
dc.contributor.authorSpeirs, V.*
dc.contributor.authorLoadman, Paul M.*
dc.contributor.authorLinforth, R.*
dc.contributor.authorKyriacou, K.*
dc.contributor.authorSutton, Chris W.*
dc.date.accessioned2016-10-11T13:26:48Z
dc.date.available2016-10-11T13:26:48Z
dc.date.issued2013
dc.identifier.citationShaheed S, Rustogi N, Scally AJ et al (2013) Identification of Stage-Specific Breast Markers Using Quantitative Proteomics. Journal of Proteome Research. 12(12): 5696-5708.en_US
dc.identifier.urihttp://hdl.handle.net/10454/9889
dc.descriptionYesen_US
dc.description.abstractMatched healthy and diseased tissues from breast cancer patients were analyzed by quantitative proteomics. By comparing proteomic profiles of fibroadenoma (benign tumors, three patients), DCIS (noninvasive cancer, three patients), and invasive ductal carcinoma (four patients), we identified protein alterations that correlated with breast cancer progression. Three 8-plex iTRAQ experiments generated an average of 826 protein identifications, of which 402 were common. After excluding those originating from blood, 59 proteins were significantly changed in tumor compared with normal tissues, with the majority associated with invasive carcinomas. Bioinformatics analysis identified relationships between proteins in this subset including roles in redox regulation, lipid transport, protein folding, and proteasomal degradation, with a substantial number increased in expression due to Myc oncogene activation. Three target proteins, cofilin-1 and p23 (increased in invasive carcinoma) and membrane copper amine oxidase 3 (decreased in invasive carcinoma), were subjected to further validation. All three were observed in phenotype-specific breast cancer cell lines, normal (nontransformed) breast cell lines, and primary breast epithelial cells by Western blotting, but only cofilin-1 and p23 were detected by multiple reaction monitoring mass spectrometry analysis. All three proteins were detected by both analytical approaches in matched tissue biopsies emulating the response observed with proteomics analysis. Tissue microarray analysis (361 patients) indicated cofilin-1 staining positively correlating with tumor grade and p23 staining with ER positive status; both therefore merit further investigation as potential biomarkers.en_US
dc.description.sponsorshipCyprus Research Promotion Foundation, Yorkshire Cancer Researchen_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttp://dx.doi.org/10.1021/pr400662ken_US
dc.subjectBreast cancer; Invasive carcinoma; DCIS; Fibroadenoma; Proteomics; iTRAQ; Mass spectrometry; Tissue microarray; Western blottingen_US
dc.titleIdentification of Stage-Specific Breast Markers using Quantitative Proteomicsen_US
dc.status.refereedYesen_US
dc.date.application2013-10-09
dc.typeArticleen_US
dc.type.versionAccepted manuscripten_US
refterms.dateFOA2018-07-25T13:08:06Z


Item file(s)

Thumbnail
Name:
shaheed_et_al_2013.pdf
Size:
1.174Mb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record