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dc.contributor.authorAyuso, J.M.*
dc.contributor.authorBasheer, Haneen A.*
dc.contributor.authorMonge, R.*
dc.contributor.authorSánchez-Álvarez, P.*
dc.contributor.authorDoblare, M.*
dc.contributor.authorShnyder, Steven*
dc.contributor.authorVinader, Victoria*
dc.contributor.authorAfarinkia, Kamyar*
dc.contributor.authorFernandez, L.J.*
dc.contributor.authorOchoa, I.*
dc.date.accessioned2016-10-11T08:27:30Z
dc.date.available2016-10-11T08:27:30Z
dc.date.issued2015-10-07
dc.identifier.citationAyuso JM, Basheer HA, Monge R et al (2015) Study of the chemotactic response of multicellular spheroids in a microfluidic device. PLoS ONE. 10(10): e0139515.
dc.identifier.urihttp://hdl.handle.net/10454/9885
dc.descriptionYes
dc.description.abstractWe report the first application of a microfluidic device to observe chemotactic migration in multicellular spheroids. A microfluidic device was designed comprising a central microchamber and two lateral channels through which reagents can be introduced. Multicellular spheroids were embedded in collagen and introduced to the microchamber. A gradient of fetal bovine serum (FBS) was established across the central chamber by addition of growth media containing serum into one of the lateral channels. We observe that spheroids of oral squamous carcinoma cells OSC–19 invade collectively in the direction of the gradient of FBS. This invasion is more directional and aggressive than that observed for individual cells in the same experimental setup. In contrast to spheroids of OSC–19, U87-MG multicellular spheroids migrate as individual cells. A study of the exposure of spheroids to the chemoattractant shows that the rate of diffusion into the spheroid is slow and thus, the chemoattractant wave engulfs the spheroid before diffusing through it.
dc.description.sponsorshipThis work has been supported by National Research Program of Spain (DPI2011-28262-c04-01) and by the project "MICROANGIOTHECAN" (CIBERBBN, IMIBIC and SEOM). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
dc.language.isoen
dc.rights© 2015 Ayuso et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.subjectChemotactic migration
dc.subjectMulticellular spheroids
dc.subjectMicrofluidic device
dc.titleStudy of the chemotactic response of multicellular spheroids in a microfluidic device
dc.status.refereedYes
dc.date.application2015-10-07
dc.typeArticle
dc.type.versionPublished version
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0139515
dc.rights.licenseCC-BY
refterms.dateFOA2018-07-25T13:10:34Z
dc.openaccess.statusopenAccess
dc.date.accepted2015-09-13


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