Show simple item record

dc.contributor.authorAyuso, J.M.
dc.contributor.authorBasheer, Haneen A.
dc.contributor.authorMonge, R.
dc.contributor.authorSánchez-Álvarez, P.
dc.contributor.authorDoblare, M.
dc.contributor.authorShnyder, Steven D.
dc.contributor.authorVinader, Victoria
dc.contributor.authorAfarinkia, Kamyar
dc.contributor.authorFernandez, L.J.
dc.contributor.authorOchoa, I.
dc.date.accessioned2016-10-11T08:27:30Z
dc.date.available2016-10-11T08:27:30Z
dc.date.issued2015-10-07
dc.identifier.citationAyuso JM, Basheer HA, Monge R et al. (2015) Study of the chemotactic response of multicellular spheroids in a microfluidic device. PLoS ONE. 10(10): e0139515.en_US
dc.identifier.urihttp://hdl.handle.net/10454/9885
dc.descriptionYesen_US
dc.description.abstractWe report the first application of a microfluidic device to observe chemotactic migration in multicellular spheroids. A microfluidic device was designed comprising a central microchamber and two lateral channels through which reagents can be introduced. Multicellular spheroids were embedded in collagen and introduced to the microchamber. A gradient of fetal bovine serum (FBS) was established across the central chamber by addition of growth media containing serum into one of the lateral channels. We observe that spheroids of oral squamous carcinoma cells OSC–19 invade collectively in the direction of the gradient of FBS. This invasion is more directional and aggressive than that observed for individual cells in the same experimental setup. In contrast to spheroids of OSC–19, U87-MG multicellular spheroids migrate as individual cells. A study of the exposure of spheroids to the chemoattractant shows that the rate of diffusion into the spheroid is slow and thus, the chemoattractant wave engulfs the spheroid before diffusing through it.en_US
dc.description.sponsorshipThis work has been supported by National Research Program of Spain (DPI2011-28262-c04-01) and by the project "MICROANGIOTHECAN" (CIBERBBN, IMIBIC and SEOM). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttp://dx.doi.org/10.1371/journal.pone.0139515en_US
dc.rights© 2015 Ayuso et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.subjectMicrofluidic device; Chemotactic migration; Multicellular spheroidsen_US
dc.titleStudy of the chemotactic response of multicellular spheroids in a microfluidic deviceen_US
dc.status.refereedYesen_US
dc.date.Accepted2015-09-13
dc.date.application2015-10-07
dc.typeArticleen_US
dc.type.versionpublished version paperen_US
refterms.dateFOA2018-07-25T13:10:34Z


Item file(s)

Thumbnail
Name:
Vinader_PLoS_ONE.pdf
Size:
4.243Mb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record