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    Study of the chemotactic response of multicellular spheroids in a microfluidic device

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    Publication date
    2015-10-07
    Author
    Ayuso, J.M.
    Basheer, Haneen A.
    Monge, R.
    Sánchez-Álvarez, P.
    Doblare, M.
    Shnyder, Steven D.
    Vinader, Victoria
    Afarinkia, Kamyar
    Fernandez, L.J.
    Ochoa, I.
    Keyword
    Microfluidic device; Chemotactic migration; Multicellular spheroids
    Rights
    © 2015 Ayuso et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
    Peer-Reviewed
    Yes
    
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    Abstract
    We report the first application of a microfluidic device to observe chemotactic migration in multicellular spheroids. A microfluidic device was designed comprising a central microchamber and two lateral channels through which reagents can be introduced. Multicellular spheroids were embedded in collagen and introduced to the microchamber. A gradient of fetal bovine serum (FBS) was established across the central chamber by addition of growth media containing serum into one of the lateral channels. We observe that spheroids of oral squamous carcinoma cells OSC–19 invade collectively in the direction of the gradient of FBS. This invasion is more directional and aggressive than that observed for individual cells in the same experimental setup. In contrast to spheroids of OSC–19, U87-MG multicellular spheroids migrate as individual cells. A study of the exposure of spheroids to the chemoattractant shows that the rate of diffusion into the spheroid is slow and thus, the chemoattractant wave engulfs the spheroid before diffusing through it.
    URI
    http://hdl.handle.net/10454/9885
    Version
    published version paper
    Citation
    Ayuso JM, Basheer HA, Monge R et al. (2015) Study of the chemotactic response of multicellular spheroids in a microfluidic device. PLoS ONE. 10(10): e0139515.
    Link to publisher’s version
    http://dx.doi.org/10.1371/journal.pone.0139515
    Type
    Article
    Collections
    Life Sciences Publications

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