Nuclear targeting of dystroglycan promotes the expression of androgen regulated transcription factors in prostate cancer
Publication date
2013Author
Mathew, G.Mitchell, Andrew
Down, J.M.
Jacobs, L.A.
Hamdy, F.C.
Eaton, C.
Rosario, D.J.
Cross, S.S.
Winder, S.J.
Keyword
AndrogensCell Line
Cell nucleus
Dystroglycans
Gene expression regulation
Humans
Immunohistochemistry
Male
Myristic acid
Oligonucleotide array sequence analysis
Phosphorylation
Phosphotyrosine
Prostate
Prostatic neoplasms
Protein transport
Transcription factors
Transcription
Open Access status
closedAccess
Metadata
Show full item recordAbstract
Dystroglycan is frequently lost in adenocarcinoma, but the mechanisms and consequences are poorly understood. We report an analysis of beta-dystroglycan in prostate cancer in human tissue samples and in LNCaP cells in vitro. There is progressive loss of beta-dystroglycan immunoreactivity from basal and lateral surfaces of prostate epithelia which correlates significantly with increasing Gleason grade. In about half of matched bone metastases there is significant dystroglycan re-expression. In tumour tissue and in LNCaP cells there is also a tyrosine phosphorylation-dependent translocation of beta-dystroglycan to the nucleus. Analysis of gene expression data by microarray, reveals that nuclear targeting of beta-dystroglycan in LNCaP cells alters the transcription of relatively few genes, the most unregulated being the transcription factor ETV1. These data suggest that proteolysis, tyrosine phosphorylation and translocation of dystroglycan to the nucleus resulting in altered gene transcription could be important mechanisms in the progression of prostate cancer.Version
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Mathew G, Mitchell A, Down JM et al (2013) Nuclear targeting of dystroglycan promotes the expression of androgen regulated transcription factors in prostate cancer. Scientific Reports, 3. Article Number 2792.Link to Version of Record
https://doi.org/10.1038/srep02792Type
Articleae974a485f413a2113503eed53cd6c53
https://doi.org/10.1038/srep02792