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dc.contributor.authorNajafzadeh, Mojgan*
dc.contributor.authorNormington, Charmaine*
dc.contributor.authorJacob, B.K.*
dc.contributor.authorIsreb, Mohammad*
dc.contributor.authorGopalan, Rajendran C.*
dc.contributor.authorAnderson, Diana*
dc.date.accessioned2016-09-29T13:51:58Z
dc.date.available2016-09-29T13:51:58Z
dc.date.issued2016-09-28
dc.identifier.citationNajafzadeh M, Normington C, Jacob BK, Isreb M, Gopala RC and Anderson D (2016) DNA Damage in Healthy Individuals and Respiratory Patients after Treating Whole Blood In vitro with the Bulk and Nano Forms of NSAIDs. Frontiers in Molecular Biosciences – Nanobiotechnology. 3: Article 50.en_US
dc.identifier.urihttp://hdl.handle.net/10454/9492
dc.descriptionYesen_US
dc.description.abstractNon-steroidal anti-inflammatory drugs (NSAIDs) inhibit COX enzyme activity which affects the inflammatory response. Inflammation is associated with increasing cancer incidence. Pre-clinical and clinical studies have shown that NSAID treatment could cause an anti-tumor effect in cancers. In the present study, blood was taken from healthy individuals (n = 17) and patients with respiratory diseases or lung cancer (n = 36). White blood cells (WBC) were treated with either a micro-suspension, i.e., bulk (B) or nano-suspension (N) of aspirin (ASP) or ibuprofen (IBU) up to 500 μg/ml in the comet assay and up to 125 μg/ml in the micronucleus assay. In this study results were compared against untreated lymphocytes and their corresponding treated groups. The results showed, that NSAIDs in their nano form significantly reduced the DNA damage in WBCs from lung cancer patients in bulk and nano compared to untreated lymphocytes. Also, there was a decrease in the level of DNA damage in the comet assay after treating WBCs from healthy individuals, asthma and COPD groups with aspirin N (ASP N) but not with IBU N. In addition, the number of micronuclei decreased after treatment with NSAIDs in their nano form (ASP N and IBU N) in the healthy as well as in the lung cancer group. However, this was not the case for micronucleus frequency in asthma and COPD patients. These data show that lymphocytes from different groups respond differently to treatment with ASP and IBU as measured by comet assay and micronucleus assay, and that the size of the suspended particles of the drugs affects responses.en_US
dc.description.sponsorshipThe present study was part funded by United Kingdom India Education Research Initiative (UKERI) SA 07-067.en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttp://dx.doi.org/10.3389/fmolb.2016.00050en_US
dc.rights© 2016 The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License CC-BY (http://creativecommons.org/licenses/by/4.0/). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en_US
dc.subjectDNA damage; Lung cancer; COPD; Asthma; Nanoparticles; Bulk forms aspirin; Ibuprofenen_US
dc.titleDNA Damage in Healthy Individuals and Respiratory Patients after Treating Whole Blood In vitro with the Bulk and Nano Forms of NSAIDsen_US
dc.status.refereedYesen_US
dc.date.Accepted2016-08-23
dc.typeArticleen_US
dc.type.versionpublished version paperen_US
refterms.dateFOA2018-07-25T14:33:46Z


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