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dc.contributor.authorZhang, J.N.*
dc.contributor.authorCui, Z.F.*
dc.contributor.authorField, R.*
dc.contributor.authorMoloney, M.G.*
dc.contributor.authorRimmer, Stephen*
dc.contributor.authorYe, H.*
dc.date.accessioned2016-09-21T17:20:47Z
dc.date.available2016-09-21T17:20:47Z
dc.date.issued2015-06
dc.identifier.citationZhang JN, Cui ZF, Field R, Moloney MG, Rimmer S and Ye H (2015) Thermo-responsive microcarriers based on poly(N-isopropylacrylamide). European Polymer Journal. 67: 346-364.
dc.identifier.urihttp://hdl.handle.net/10454/9425
dc.descriptionNoen
dc.description.abstractMicrocarrier cell culture systems provide an attractive alternative to the conventional monolayer cell culture for cell amplification, due to their high surface area-to-volume ratio. Unlike enzymatic methods for removing cells from microcarriers after cell culture, which can lead to irreversible damage of the cells, microcarriers which release cells by temperature adjustment have been developed. This was achieved by grafting a temperature-responsive polymer, poly(N-isopropylacrylamide) (PNIPAAm), on the microcarrier surface. This review comprehensively presents various methods to prepare such thermo-responsive microcarriers based on PNIPAAm. These methods include the grafting-to technique, grafting-from technique, grafting-through technique, along with methods leading to PNIPAAm hydrogel beads, seeded polymerization, and non-covalent adsorption. The methods for controlling PNIPAAm grafting density, molecular weight and molecular architecture are also outlined. Further, the efficiency of cell attachment, proliferation and thermally-induced detachment of such thermo-responsive microcarriers is introduced and compared. (C) 2015 Elsevier Ltd. All rights reserved.
dc.description.sponsorshipEPSRC
dc.relation.isreferencedbyhttp://dx.doi.org/10.1016/j.eurpolymj.2015.04.013
dc.subjectThermo-responsive microcarrier
dc.subject; Poly(n-isopropylaciylamide)
dc.subject; Cell culture
dc.subject; Polymerisation
dc.subject; Transfer radical polymerization
dc.subject; Surface-initiated atrp
dc.subject; Membrane emulsification technique
dc.subject; Polystyrene latex-particles
dc.subject; Thermally reversible hydrogel
dc.subject; Core-shell microcapsules
dc.subject; Narrow size distribution
dc.subject; Modulated skin layers
dc.subject; Embryonic stem-cells
dc.subject; Cross-flow membrane
dc.titleThermo-responsive microcarriers based on poly(N-isopropylacrylamide)
dc.date.Accepted2015-04-10
dc.date.application2015-04-17
dc.typeArticle
dc.type.versionNo full-text in the repositoryen


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