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dc.contributor.authorUmerska, A.*
dc.contributor.authorPaluch, Krzysztof J.*
dc.contributor.authorSantos-Martinez, M.J.*
dc.contributor.authorMedina, C.*
dc.contributor.authorCorrigan, O.I.*
dc.contributor.authorTajber, L.*
dc.date.accessioned2016-09-21T17:19:34Z
dc.date.available2016-09-21T17:19:34Z
dc.date.issued2015-06
dc.identifier.citationUmerska A, Paluch KJ, Santos-Martinez MJ et al . (2015) Chondroitin-based nanoplexes as peptide delivery systems - Investigations into the self-assembly process, solid-state and extended release characteristics. European Journal of Pharmaceutics and Biopharmaceutics. 93: 242-253.
dc.identifier.urihttp://hdl.handle.net/10454/9416
dc.descriptionYes
dc.description.abstractA new type of self-assembled polyelectrolyte complex nanocarrier composed of chondroitin (CHON) and protamine (PROT) was designed and the ability of the carriers to bind salmon calcitonin (sCT) was examined. The response of sCT-loaded CHON/PROT NPs to a change in the properties of the liquid medium, e.g. its pH, composition or ionic strength was studied and in vitro peptide release was assessed. The biocompatibility of the NPs was evaluated in Caco-2 cells. CHON/PROT NPs were successfully obtained with properties that were dependent on the concentration of the polyelectrolytes and their mixing ratio. X-ray diffraction determined the amorphous nature of the negatively charged NPs, while those with the positive surface potential were semi-crystalline. sCT was efficiently associated with the nanocarriers (98-100%) and a notably high drug loading (13-38%) was achieved. The particles had negative zeta potential values and were homogenously dispersed with sizes between 60 and 250 nm. CHON/PROT NPs released less than 10% of the total loaded peptide in the first hour of the in vitro release studies. The enthalpy of the decomposition exotherm correlated with the amount of sCT remaining in NPs after the release experiments. The composition of medium and its ionic strength was found to have a considerable influence on the release of sCT from CHON/PROT NPs. Complexation to CHON markedly reduced the toxic effects exerted by PROT and the NPs were compatible and well tolerated by Caco-2 cells.
dc.rights© 2015 Elsevier. Reproduced in accordance with the publisher's self-archiving policy. This manuscript version is made available under the CC-BY-NC-ND 4.0 license.
dc.subjectCaco-2 cells: Calcitonin, Administration & dosage; Metabolism: Cell Survival; Pharmaceutical; Chondroitin; Toxicity: Crystallography, X-Ray; Delayed-action preparations; Drug carriers; Humans; Hydrogen-ion concentration; Kinetics; Nanomedicine; Nanoparticles; Osmolar concentration: Protamines, chemistry & metabolism; Protein binding; Solubility; Surface properties; Technology; Pharmaceutical methods; Biocompatibility; Peptide delivery; Polyelectrolyte complex; Protamine toxicity
dc.titleChondroitin-based nanoplexes as peptide delivery systems-Investigations into the self-assembly process, solid-state and extended release characteristics
dc.status.refereedyes
dc.date.Accepted2015-04-13
dc.date.application2015-04-20
dc.typeJournal Article
dc.type.versionAccepted Manuscript
dc.identifier.doihttps://doi.org/10.1016/j.ejpb.2015.04.006
refterms.dateFOA2018-07-25T14:28:21Z


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