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dc.contributor.authorPateman, C.J.*
dc.contributor.authorHarding, A.J.*
dc.contributor.authorGlen, A.*
dc.contributor.authorTaylor, C.S.*
dc.contributor.authorChristmas, C.R.*
dc.contributor.authorRobinson, P.P.*
dc.contributor.authorRimmer, Stephen*
dc.contributor.authorBoissonade, F.M.*
dc.contributor.authorClaeyssens, F.*
dc.contributor.authorHaycock, J.W.*
dc.date.accessioned2016-09-21T17:18:42Z
dc.date.available2016-09-21T17:18:42Z
dc.date.issued2015
dc.identifier.citationPateman CJ, Harding AJ, Glen A et al (2015) Nerve guides manufactured from photocurable polymers to aid peripheral nerve repair. Biomaterials. 49: 77-89.
dc.identifier.urihttp://hdl.handle.net/10454/9391
dc.descriptionYes
dc.description.abstractThe peripheral nervous system has a limited innate capacity for self-repair following injury, and surgical intervention is often required. For injuries greater than a few millimeters autografting is standard practice although it is associated with donor site morbidity and is limited in its availability. Because of this, nerve guidance conduits (NGCs) can be viewed as an advantageous alternative, but currently have limited efficacy for short and large injury gaps in comparison to autograft. Current commercially available NGC designs rely on existing regulatory approved materials and traditional production methods, limiting improvement of their design. The aim of this study was to establish a novel method for NGC manufacture using a custom built laser-based microstereolithography (muSL) setup that incorporated a 405 nm laser source to produce 3D constructs with approximately 50 mum resolution from a photocurable poly(ethylene glycol) resin. These were evaluated by SEM, in vitro neuronal, Schwann and dorsal root ganglion culture and in vivo using a thy-1-YFP-H mouse common fibular nerve injury model. NGCs with dimensions of 1 mm internal diameter x 5 mm length with a wall thickness of 250 mum were fabricated and capable of supporting re-innervation across a 3 mm injury gap after 21 days, with results close to that of an autograft control. The study provides a technology platform for the rapid microfabrication of biocompatible materials, a novel method for in vivo evaluation, and a benchmark for future development in more advanced NGC designs, biodegradable and larger device sizes, and longer-term implantation studies.
dc.relation.isreferencedbyhttp://dx.doi.org/10.1016/j.biomaterials.2015.01.055
dc.rights(c) 2015 The Authors. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)
dc.subjectAnimals
dc.subject; Axons
dc.subject; Biocompatible materials
dc.subject; Cells
dc.subject; Compressive strength
dc.subject; Disease models
dc.subject; Fibula
dc.subject; Ganglia
dc.subject; Guided tissue regeneration
dc.subject; Materials testing
dc.subject; Mice
dc.subject; Microscopy
dc.subject; Nerve regeneration
dc.subject; Peripheral nerves
dc.subject; Photochemical processes
dc.subject; Polyethylene glycols
dc.subject; Printing
dc.subject; Prosthesis implantation
dc.subject; Rats
dc.subject; Wound healing
dc.subject; Microstructure
dc.subject; Nerve guide
dc.subject; Nerve regeneration
dc.subject; Nerve tissue engineering
dc.subject; Neural cell
dc.subject; Schwann cell
dc.titleNerve guides manufactured from photocurable polymers to aid peripheral nerve repair
dc.status.refereedYes
dc.date.Accepted2015-01-20
dc.date.application2015-02-14
dc.typeArticle
dc.type.versionPublished version
refterms.dateFOA2018-07-25T14:26:59Z


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