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dc.contributor.authorKhan, F.A.*
dc.contributor.authorKhan, N.M.*
dc.contributor.authorKhan, H.U.*
dc.contributor.authorKhan, S.*
dc.contributor.authorAli, N.*
dc.contributor.authorAhmad, S.*
dc.contributor.authorMaitland, Derek J.*
dc.date.accessioned2016-09-21T17:17:34Z
dc.date.available2016-09-21T17:17:34Z
dc.date.issued2015
dc.identifier.citationKhan FA, Khan NM, Khan HU et al (2015) Buddlejol, a new alpha-chymotrypsin inhibitor from Buddleja asiatica. Medicinal Chemistry Research. 24(3): 980-986.
dc.identifier.urihttp://hdl.handle.net/10454/9366
dc.descriptionNo
dc.description.abstractBuddlejol (1), a new sterol, has been isolated from the ethyl acetate soluble fraction of the antispasmodic plant Buddleja asiatica along with stigmasterol (2), lignoceric acid (3), taraxerol (4) and alpha-amyrin (5), respectively. The structure of Buddlejol (1) was established as (24S)-stigmast-5,22-diene-7 beta-ethoxy-3 beta-ol by spectral analysis and comparison with closely related structures. Buddlejol revealed to be a competitive inhibitor of chymotrypsin with the Ki value of 10.60 A mu M as indicated by Lineweaver-Burk and Dixon plots and their re-plots against its chymotrypsin inhibition assay, while the other compounds showed less inhibitory potential. The bioassay-guided isolation was stimulated by the preliminary cytotoxic screening of various fractions of B. asiatica.
dc.language.isoen
dc.subjectBuddleja asiatica
dc.subjectEthyl acetate soluble fraction
dc.subjectBuddlejol
dc.subjectCytotoxic activity
dc.subjectChymotrypsin inhibition
dc.subjectChemical-constituents
dc.subjectMedicinal-plants
dc.subjectMechanism
dc.subjectElastase
dc.subjectTobacco
dc.subjectChina
dc.subjectLour
dc.titleBuddlejol, a new alpha-chymotrypsin inhibitor from Buddleja asiatica
dc.status.refereedYes
dc.typeArticle
dc.type.versionNo full-text in the repository
dc.identifier.doihttps://doi.org/10.1007/s00044-014-1192-9
dc.openaccess.statusclosedAccess


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