Buddlejol, a new alpha-chymotrypsin inhibitor from Buddleja asiatica
Publication date
2015Keyword
Buddleja asiaticaEthyl acetate soluble fraction
Buddlejol
Cytotoxic activity
Chymotrypsin inhibition
Chemical-constituents
Medicinal-plants
Mechanism
Elastase
Tobacco
China
Lour
Peer-Reviewed
YesOpen Access status
closedAccess
Metadata
Show full item recordAbstract
Buddlejol (1), a new sterol, has been isolated from the ethyl acetate soluble fraction of the antispasmodic plant Buddleja asiatica along with stigmasterol (2), lignoceric acid (3), taraxerol (4) and alpha-amyrin (5), respectively. The structure of Buddlejol (1) was established as (24S)-stigmast-5,22-diene-7 beta-ethoxy-3 beta-ol by spectral analysis and comparison with closely related structures. Buddlejol revealed to be a competitive inhibitor of chymotrypsin with the Ki value of 10.60 A mu M as indicated by Lineweaver-Burk and Dixon plots and their re-plots against its chymotrypsin inhibition assay, while the other compounds showed less inhibitory potential. The bioassay-guided isolation was stimulated by the preliminary cytotoxic screening of various fractions of B. asiatica.Version
No full-text in the repositoryCitation
Khan FA, Khan NM, Khan HU et al (2015) Buddlejol, a new alpha-chymotrypsin inhibitor from Buddleja asiatica. Medicinal Chemistry Research. 24(3): 980-986.Link to Version of Record
https://doi.org/10.1007/s00044-014-1192-9Type
Articleae974a485f413a2113503eed53cd6c53
https://doi.org/10.1007/s00044-014-1192-9