Show simple item record

dc.contributor.authorSefat, Farshid
dc.contributor.authorYouseffi, Mansour
dc.contributor.authorKhaghani, Seyed A.
dc.contributor.authorSoon, Chin Fhong
dc.contributor.authorJavid, Farideh A.
dc.date.accessioned2016-09-05T12:53:12Z
dc.date.available2016-09-05T12:53:12Z
dc.date.issued2016-07
dc.identifier.citationSefat F, Youseffi M, Khaghani SA, Soon CF and Javid F (2016) Effect of Transforming Growth Factor-β3 on mono and multilayer chondrocytes. Cytokine. 83: 118-126.en_US
dc.identifier.urihttp://hdl.handle.net/10454/8908
dc.descriptionYesen_US
dc.description.abstractArticular cartilage is an avascular and flexible connective tissue found in joints. It produces a cushioning effect at the joints and provides low friction to protect the ends of the bones from wear and tear/damage. It has poor repair capacity and any injury can result pain and loss of mobility. Transforming growth factor-beta (TGF-β), a cytokine superfamily, regulates cell function, including differentiation and proliferation. Although the function of the TGF-βs in various cell types has been investigated, their function in cartilage repair is as yet not fully understood. The effect of TGF-β3 in biological regulation of primary chondrocyte was investigated in this work. TGF-β3 provided fibroblastic morphology to chondrocytes and therefore overall reduction in cell proliferation was observed. The length of the cells supplemented with TGF-β3 were larger than the cells without TGF-β3 treatment. This was caused by the fibroblast like cells (dedifferentiated chondrocytes) which occupied larger areas compared to cells without TGF-β3 addition. The healing process of the model wound closure assay of chondrocyte multilayer was slowed down by TGF-β3, and this cytokine negatively affected the strength of chondrocyte adhesion to the cell culture surface.en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttp://dx.doi.org/10.1016/j.cyto.2016.04.008en_US
dc.rights© 2016 Elsevier, Ltd. Reproduced in accordance with the publisher's self-archiving policy. This manuscript version is made available under the CC-BY-NC-ND 4.0 license (http://creativecommons.org/licenses/by-nc-nd/4.0/)en_US
dc.subjectCell length; Cell proliferation; Model wound closure assay; Primary chondrocyte multilayer; TGF-β3en_US
dc.titleEffect of Transforming Growth Factor-β3 on mono and multilayer chondrocytesen_US
dc.status.refereedYesen_US
dc.date.Accepted2016-04-18
dc.date.application2016-04-22
dc.typeArticleen_US
dc.type.versionAccepted manuscripten_US
refterms.dateFOA2018-07-25T14:58:09Z


Item file(s)

Thumbnail
Name:
cytokine_2016.pdf
Size:
1.487Mb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record