An optimised assay for quantitative, high-throughput analysis of polysialyltransferase activity
dc.contributor.author | Elkashef, Sara M. | * |
dc.contributor.author | Sutherland, Mark | * |
dc.contributor.author | Patterson, Laurence H. | * |
dc.contributor.author | Loadman, Paul | * |
dc.contributor.author | Falconer, Robert A. | * |
dc.date.accessioned | 2016-08-15T11:05:45Z | |
dc.date.available | 2016-08-15T11:05:45Z | |
dc.date.issued | 2016-08-08 | |
dc.identifier.citation | Elkashef SM, Sutherland M, Patterson LH, Loadman PM and Falconer RA (2016) An optimised assay for quantitative, high-throughput analysis of polysialyltransferase activity. Analyst. 141(20): 5849-5856. | en_US |
dc.identifier.uri | http://hdl.handle.net/10454/8803 | |
dc.description | Yes | en_US |
dc.description.abstract | The polysialyltransferases are biologically important glycosyltransferase enzymes responsible for the biosynthesis of polysialic acid, a carbohydrate polymer that plays a critical role in the progression of several diseases, notably cancer. Having improved the chemical synthesis and purification of the fluorescently-labelled DMB-DP3 acceptor, we report optimisation and validation of a highly sensitive cell-free high-throughput HPLC-based assay for assessment of human polysialyltransferase activity. | en_US |
dc.language.iso | en | en_US |
dc.rights | © 2016 Royal Society of Chemistry. Full-text reproduced in accordance with the publisher’s self-archiving policy. | en_US |
dc.subject | Polysialytransferases; Glycosyltransferase enzymes; Polysialic acid; Carbohydrate polymers; Cancer; DMB-DP3 acceptor; Assay | en_US |
dc.title | An optimised assay for quantitative, high-throughput analysis of polysialyltransferase activity | en_US |
dc.status.refereed | Yes | en_US |
dc.date.Accepted | 2016-08-07 | |
dc.type | Article | en_US |
dc.type.version | Accepted Manuscript | en_US |
dc.identifier.doi | https://doi.org/10.1039/C6AN01073C |