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dc.contributor.authorElkashef, Sara M.
dc.contributor.authorSutherland, Mark H.
dc.contributor.authorPatterson, Laurence H.
dc.contributor.authorLoadman, Paul M.
dc.contributor.authorFalconer, Robert A.
dc.date.accessioned2016-08-15T11:05:45Z
dc.date.available2016-08-15T11:05:45Z
dc.date.issued2016-08-08
dc.identifier.citationElkashef SM, Sutherland M, Patterson LH, Loadman PM and Falconer RA (2016) An optimised assay for quantitative, high-throughput analysis of polysialyltransferase activity. Analyst. 141(20): 5849-5856.en_US
dc.identifier.urihttp://hdl.handle.net/10454/8803
dc.descriptionYesen_US
dc.description.abstractThe polysialyltransferases are biologically important glycosyltransferase enzymes responsible for the biosynthesis of polysialic acid, a carbohydrate polymer that plays a critical role in the progression of several diseases, notably cancer. Having improved the chemical synthesis and purification of the fluorescently-labelled DMB-DP3 acceptor, we report optimisation and validation of a highly sensitive cell-free high-throughput HPLC-based assay for assessment of human polysialyltransferase activity.en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttp://dx.doi.org/10.1039/C6AN01073Cen_US
dc.rights© 2016 Royal Society of Chemistry. Full-text reproduced in accordance with the publisher’s self-archiving policy.en_US
dc.subjectPolysialytransferases; Glycosyltransferase enzymes; Polysialic acid; Carbohydrate polymers; Cancer; DMB-DP3 acceptor; Assayen_US
dc.titleAn optimised assay for quantitative, high-throughput analysis of polysialyltransferase activityen_US
dc.status.refereedYesen_US
dc.date.Accepted2016-08-07
dc.typeArticleen_US
dc.type.versionAccepted Manuscripten_US


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