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dc.contributor.authorAl balkhi, M.H.*
dc.contributor.authorMohammad, Mohammad A.*
dc.contributor.authorTisserant, L-P.*
dc.contributor.authorBoitel-Conti, M.*
dc.date.accessioned2016-07-27T08:20:34Z
dc.date.available2016-07-27T08:20:34Z
dc.date.issued2016-10
dc.identifier.citationAl balkhi MH, Mohammad MA, Tisserant L-P and Boitel-Conti M (2016) Development of a liquid-liquid extraction method of resveratrol from cell culture media using solubility parameters. Separation and Purification Technology. 170: 138-145.en_US
dc.identifier.urihttp://hdl.handle.net/10454/8703
dc.descriptionYesen_US
dc.description.abstractThe extraction of bioactive compounds, produced by plant cell cultures, directly from their culture medium, which contains other by-products, is a great challenge. Resveratrol extraction from its grapevine cell cultures is considered here as an example to improve the extraction processes from plant cell cultures using solubility parameters. Successive liquid-liquid extraction (LLE) processes were exploited to extract resveratrol from the culture medium with an extraction ratio approaching 100%, high selectivity and minimum amounts of solvents. The calculations of partition coefficients as a function of solubility parameters demonstrated that benzyl benzoate is the most suitable intermediate solvent to extract resveratrol from its aqueous medium. The calculations also illustrated the high ability of methanol and ethanol to extract resveratrol from benzyl benzoate. The physicochemical properties of benzyl benzoate and processing conditions were exploited to separate it from aqueous media and organic solvents. The agitation method, component ratios and extraction time were studied to maximize the extraction yield. Under the best studied conditions, the recovery of resveratrol from different culture media approached ∼100% with a selectivity of ∼92%. Ultimately, the improved extraction processes of resveratrol are markedly efficient, selective, rapid and economical.en_US
dc.description.sponsorshipMohammad Amin Mohammad gratefully acknowledges CARA (The Council for At-Risk Academics, Stephen Wordsworth and Ryan Mundy) for providing the financial support for an academic fellowship.en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttp://dx.doi.org/10.1016/j.seppur.2016.06.037en_US
dc.rights© 2016 Elsevier B. V. Reproduced in accordance with the publisher's self-archiving policy. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/en_US
dc.subjectBenzyl benzoate; Liquid-liquid extraction; Partition coefficient; Plant cell culture; Resveratrol; Solubility parameters; Solvent consumptionen_US
dc.titleDevelopment of a liquid-liquid extraction method of resveratrol from cell culture media using solubility parametersen_US
dc.status.refereedYesen_US
dc.date.Accepted2016-06-22
dc.date.application2016-06-23
dc.typeArticleen_US
dc.type.versionAccepted Manuscripten_US
refterms.dateFOA2018-07-25T14:56:34Z


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