Show simple item record

dc.contributor.authorSoon, Chin Fhong*
dc.contributor.authorOmar, W.I.W.*
dc.contributor.authorBerends, Rebecca F.*
dc.contributor.authorNayan, N.*
dc.contributor.authorBasri, H.*
dc.contributor.authorTee, K.S.*
dc.contributor.authorYouseffi, Mansour*
dc.contributor.authorBlagden, Nicholas*
dc.contributor.authorDenyer, Morgan C.T.*
dc.date.accessioned2016-06-27T13:50:22Z
dc.date.available2016-06-27T13:50:22Z
dc.date.issued2014-01
dc.identifier.citationSoon CF, Omar WIW, Berends RF et al (2014) Biophysical characteristics of cells cultured on cholesteryl ester liquid crystals. Micron 56: 73-79.en_US
dc.identifier.urihttp://hdl.handle.net/10454/8565
dc.descriptionnoen_US
dc.description.abstractThis study aimed at examining the biophysical characteristics of human derived keratinocytes (HaCaT) cultured on cholesteryl ester liquid crystals (CELC). CELC was previously shown to improve sensitivity in sensing cell contractions. Characteristics of the cell integrin expressions and presence of extracellular matrix (ECM) proteins on the liquid crystals were interrogated using various immunocytochemical techniques. The investigation was followed by characterization of the chemical properties of the liquid crystals (LC) after immersion in cell culture media using Fourier transform infrared spectroscopy (FTIR). The surface morphology of cells adhered to the LC was studied using atomic force microscopy (AFM). Consistent with the expressions of the integrins α2, α3 and β1, extracellular matrix proteins (laminin, collagen type IV and fibronectin) were found secreted by the HaCaT onto CELC and these proteins were also secreted by cells cultured on the glass substrates. FTIR analysis of the LC revealed the existence of spectrum assigned to cholesterol and ester moieties that are essential compounds for the metabolizing activities of keratinocytes. The immunostainings indicated that cell adhesion on the LC is mediated by self-secreted ECM proteins. As revealed by the AFM imaging, the constraint in cell membrane spread on the LC leads to the increase in cell surface roughness and thickness of cell membrane. The biophysical expressions of cells on biocompatible CELC suggested that CELC could be a new class of biological relevant material.en_US
dc.language.isoenen_US
dc.rights© 2014 Elsevier. Reproduced in accordance with the publisher's self-archiving policy. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/en_US
dc.subjectKeratinocyte; Liquid crystals; Cell adhesion; Cell surface morphology; AFM; FTIR; Integrin; Cell surface roughness; Cholesteryl ester; Extracellular matrix proteinsen_US
dc.titleBiophysical characteristics of cells cultured on cholesteryl ester liquid crystalsen_US
dc.status.refereedyesen_US
dc.date.Accepted2013-10-14
dc.typeArticleen_US
dc.type.versionNo full-text available in the repositoryen_US
dc.identifier.doihttps://doi.org/10.1016/j.micron.2013.10.011


Item file(s)

Thumbnail
Name:
JPaperSoonMYMD-2014-Micron.pdf
Size:
1.637Mb
Format:
PDF
Description:
Suppressed - published version

This item appears in the following Collection(s)

Show simple item record