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    Biophysical characteristics of cells cultured on cholesteryl ester liquid crystals

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    Publication date
    2014-01
    Author
    Soon, Chin Fhong
    Omar, W.I.W.
    Berends, Rebecca F.
    Nayan, N.
    Basri, H.
    Tee, K.S.
    Youseffi, Mansour
    Blagden, Nicholas
    Denyer, Morgan C.T.
    Keyword
    Keratinocyte; Liquid crystals; Cell adhesion; Cell surface morphology; AFM; FTIR; Integrin; Cell surface roughness; Cholesteryl ester; Extracellular matrix proteins
    Rights
    © 2014 Elsevier. Reproduced in accordance with the publisher's self-archiving policy. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
    Peer-Reviewed
    yes
    
    Metadata
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    Abstract
    This study aimed at examining the biophysical characteristics of human derived keratinocytes (HaCaT) cultured on cholesteryl ester liquid crystals (CELC). CELC was previously shown to improve sensitivity in sensing cell contractions. Characteristics of the cell integrin expressions and presence of extracellular matrix (ECM) proteins on the liquid crystals were interrogated using various immunocytochemical techniques. The investigation was followed by characterization of the chemical properties of the liquid crystals (LC) after immersion in cell culture media using Fourier transform infrared spectroscopy (FTIR). The surface morphology of cells adhered to the LC was studied using atomic force microscopy (AFM). Consistent with the expressions of the integrins α2, α3 and β1, extracellular matrix proteins (laminin, collagen type IV and fibronectin) were found secreted by the HaCaT onto CELC and these proteins were also secreted by cells cultured on the glass substrates. FTIR analysis of the LC revealed the existence of spectrum assigned to cholesterol and ester moieties that are essential compounds for the metabolizing activities of keratinocytes. The immunostainings indicated that cell adhesion on the LC is mediated by self-secreted ECM proteins. As revealed by the AFM imaging, the constraint in cell membrane spread on the LC leads to the increase in cell surface roughness and thickness of cell membrane. The biophysical expressions of cells on biocompatible CELC suggested that CELC could be a new class of biological relevant material.
    URI
    http://hdl.handle.net/10454/8565
    Version
    No full-text available in the repository
    Citation
    Soon CF, Omar WIW, Berends RF et al (2014) Biophysical characteristics of cells cultured on cholesteryl ester liquid crystals. Micron 56: 73-79.
    Link to publisher’s version
    http://dx.doi.org/10.1016/j.micron.2013.10.011
    Type
    Article
    Collections
    Life Sciences Publications
    Engineering and Informatics Publications

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