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dc.contributor.authorHaggag, Y.A.*
dc.contributor.authorAbdel-Wahab, Y.*
dc.contributor.authorOjo, O.*
dc.contributor.authorOsman, M.A.*
dc.contributor.authorEl-Gizawy, S.*
dc.contributor.authorEl-Tanani, Mohamed*
dc.contributor.authorFaheem, A.*
dc.contributor.authorMcCarron, P.A.*
dc.date.accessioned2016-05-24T10:24:12Z
dc.date.available2016-05-24T10:24:12Z
dc.date.issued2016-02-29
dc.identifier.citationHaggag Y, Abdel-Wahab Y, Ojo O et al. (2016) Preparation and in vivo evaluation of insulin-loaded biodegradable nanoparticles prepared from diblock copolymers of PLGA and PEG. International Journal of Pharmaceutics. 499(1-2): 236-246.en_US
dc.identifier.urihttp://hdl.handle.net/10454/8405
dc.descriptionYesen_US
dc.description.abstractThe aim of this study was to design a controlled release vehicle for insulin to preserve its stability and biological activity during fabrication and release. A modified, double emulsion, solvent evaporation, technique using homogenisation force optimised entrapment efficiency of insulin into biodegradable nanoparticles (NP) prepared from poly (dl-lactic-co-glycolic acid) (PLGA) and its PEGylated diblock copolymers. Formulation parameters (type of polymer and its concentration, stabiliser concentration and volume of internal aqueous phase) and physicochemical characteristics (size, zeta potential, encapsulation efficiency, in vitro release profiles and in vitro stability) were investigated. In vivo insulin sensitivity was tested by diet-induced type II diabetic mice. Bioactivity of insulin was studied using Swiss TO mice with streptozotocin-induced type I diabetic profile. Insulin-loaded NP were spherical and negatively charged with an average diameter of 200–400 nm. Insulin encapsulation efficiency increased significantly with increasing ratio of co-polymeric PEG. The internal aqueous phase volume had a significant impact on encapsulation efficiency, initial burst release and NP size. Optimised insulin NP formulated from 10% PEG–PLGA retained insulin integrity in vitro, insulin sensitivity in vivo and induced a sustained hypoglycaemic effect from 3 h to 6 days in type I diabetic mice.en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttp://dx.doi.org/10.1016/j.ijpharm.2015.12.063en_US
dc.rights© 2016 Elsevier B.V. Reproduced in accordance with the publisher's self-archiving policy. This manuscript version is made available under the the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/en_US
dc.subjectInsulin; PEG-PLGA; Nanoparticles; Stability; Controlled delivery; Diabetesen_US
dc.titlePreparation and in vivo evaluation of insulin-loaded biodegradable nanoparticles prepared from diblock copolymers of PLGA and PEGen_US
dc.status.refereedYesen_US
dc.date.Accepted2015-12-26
dc.date.application2015-12-30
dc.typeArticleen_US
dc.type.versionAccepted Manuscripten_US
refterms.dateFOA2018-07-25T14:00:30Z


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