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dc.contributor.authorJaafari, N.*
dc.contributor.authorKonopacki, F.A.*
dc.contributor.authorOwen, T.F.*
dc.contributor.authorKantamneni, Sriharsha*
dc.contributor.authorRubin, P.*
dc.contributor.authorCraig, T.J.*
dc.contributor.authorWilkinson, K.A.*
dc.contributor.authorHenley, J.M.*
dc.date.accessioned2016-04-13T15:35:50Z
dc.date.available2016-04-13T15:35:50Z
dc.date.issued2013-01-11
dc.identifier.citationJaafari N, Konopacki FA, Owen TF, Kantamneni S, Rubin P, Craig TJ, Wilkinson KA, Henley JM (2013) SUMOylation Is Required for Glycine-Induced Increases in AMPA Receptor Surface Expression (ChemLTP) in Hippocampal Neurons. PLoS ONE 8(1): e52345.en_US
dc.identifier.urihttp://hdl.handle.net/10454/8124
dc.descriptionyesen_US
dc.description.abstractMultiple pathways participate in the AMPA receptor trafficking that underlies long-term potentiation (LTP) of synaptic transmission. Here we demonstrate that protein SUMOylation is required for insertion of the GluA1 AMPAR subunit following transient glycine-evoked increase in AMPA receptor surface expression (ChemLTP) in dispersed neuronal cultures. ChemLTP increases co-localisation of SUMO-1 and the SUMO conjugating enzyme Ubc9 and with PSD95 consistent with the recruitment of SUMOylated proteins to dendritic spines. In addition, we show that ChemLTP increases dendritic levels of SUMO-1 and Ubc9 mRNA. Consistent with activity dependent translocation of these mRNAs to sites near synapses, levels of the mRNA binding and dendritic transport protein CPEB are also increased by ChemLTP. Importantly, reducing the extent of substrate protein SUMOylation by overexpressing the deSUMOylating enzyme SENP-1 or inhibiting SUMOylation by expressing dominant negative Ubc9 prevent the ChemLTP-induced increase in both AMPAR surface expression and dendritic SUMO-1 mRNA. Taken together these data demonstrate that SUMOylation of synaptic protein(s) involved in AMPA receptor trafficking is necessary for activity-dependent increases in AMPAR surface expression.en_US
dc.description.sponsorshipMedical Research Council, the European Research Council and the Wellcome Trusten_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttp://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0052345en_US
dc.rights© 2016 The Authors. Published by PLOS One Reproduced in accordance with the publisher's self-archiving policy, under a Creative Commons attribution licence.en_US
dc.subjectSUMO, SENP, AMPA Receptor, Surface Expression, ChemLTP, SUMOylationen_US
dc.titleSUMOylation Is Required for Glycine-Induced Increases in AMPA Receptor Surface Expression (ChemLTP) in Hippocampal Neuronsen_US
dc.status.refereedyesen_US
dc.date.Accepted2012-11-16
dc.typeArticleen_US
dc.type.versionpublished version paperen_US


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