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    SUMOylation Is Required for Glycine-Induced Increases in AMPA Receptor Surface Expression (ChemLTP) in Hippocampal Neurons

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    journal.pone.0052345.pdf (3.286Mb)
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    Publication date
    2013-01-11
    Author
    Jaafari, N.
    Konopacki, F.A.
    Owen, T.F.
    Kantamneni, Sriharsha
    Rubin, P.
    Craig, T.J.
    Wilkinson, K.A.
    Henley, J.M.
    Keyword
    SUMO, SENP, AMPA Receptor, Surface Expression, ChemLTP, SUMOylation
    Rights
    © 2016 The Authors. Published by PLOS One Reproduced in accordance with the publisher's self-archiving policy, under a Creative Commons attribution licence.
    Peer-Reviewed
    yes
    
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    Abstract
    Multiple pathways participate in the AMPA receptor trafficking that underlies long-term potentiation (LTP) of synaptic transmission. Here we demonstrate that protein SUMOylation is required for insertion of the GluA1 AMPAR subunit following transient glycine-evoked increase in AMPA receptor surface expression (ChemLTP) in dispersed neuronal cultures. ChemLTP increases co-localisation of SUMO-1 and the SUMO conjugating enzyme Ubc9 and with PSD95 consistent with the recruitment of SUMOylated proteins to dendritic spines. In addition, we show that ChemLTP increases dendritic levels of SUMO-1 and Ubc9 mRNA. Consistent with activity dependent translocation of these mRNAs to sites near synapses, levels of the mRNA binding and dendritic transport protein CPEB are also increased by ChemLTP. Importantly, reducing the extent of substrate protein SUMOylation by overexpressing the deSUMOylating enzyme SENP-1 or inhibiting SUMOylation by expressing dominant negative Ubc9 prevent the ChemLTP-induced increase in both AMPAR surface expression and dendritic SUMO-1 mRNA. Taken together these data demonstrate that SUMOylation of synaptic protein(s) involved in AMPA receptor trafficking is necessary for activity-dependent increases in AMPAR surface expression.
    URI
    http://hdl.handle.net/10454/8124
    Version
    published version paper
    Citation
    Jaafari N, Konopacki FA, Owen TF, Kantamneni S, Rubin P, Craig TJ, Wilkinson KA, Henley JM (2013) SUMOylation Is Required for Glycine-Induced Increases in AMPA Receptor Surface Expression (ChemLTP) in Hippocampal Neurons. PLoS ONE 8(1): e52345.
    Link to publisher’s version
    http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0052345
    Type
    Article
    Collections
    Life Sciences Publications

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