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dc.contributor.authorLento, S.
dc.contributor.authorBrioschi, M.
dc.contributor.authorBarcella, S.
dc.contributor.authorNasim, Md. Talat
dc.contributor.authorGhilardi, S.
dc.contributor.authorBarbieri, S.S.
dc.contributor.authorTremoli, E.
dc.contributor.authorBanfi, C.
dc.date.accessioned2016-03-23T09:48:45Z
dc.date.available2016-03-23T09:48:45Z
dc.date.issued2015-04-24
dc.identifier.citationLento S, Brioschi M, Barcella S, Nasim MT et al (2015) Proteomics of tissue factor silencing in cardiomyocytic cells reveals a new role for this coagulation factor in splicing machinery control. Journal of Proteomics. 119: 75-89.en_US
dc.identifier.urihttp://hdl.handle.net/10454/8001
dc.descriptionYesen_US
dc.description.abstractIt has long been known that Tissue Factor (TF) plays a role in blood coagulation and has a direct thrombotic action that is closely related to cardiovascular risk, but it is becoming increasingly clear that it has a much wider range of biological functions that range from inflammation to immunity. It is also involved in maintaining heart haemostasis and structure, and the observation that it is down-regulated in the myocardium of patients with dilated cardiomyopathy suggests that it influences cell-to-cell contact stability and contractility, and thus contributes to cardiac dysfunction. However, the molecular mechanisms underlying these coagulation-independent functions have not yet been fully elucidated. In order to analyse the influence of TF on the cardiomyocitic proteome, we used functional biochemical approaches incorporating label-free quantitative proteomics and gene silencing, and found that this provided a powerful means of identifying a new role for TF in regulating splicing machinery together with the expression of several proteins of the spliceosome, and mRNA metabolism with a considerable impact on cell viability.en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttp://dx.doi.org/10.1016/j.jprot.2015.01.021en_US
dc.rights© 2015 Elsevier B.V. Full-text reproduced in accordance with the publisher’s self-archiving policy. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/en_US
dc.subjectTissue factor; Proteomics; Spliceosome; Splicing; Cardiomyocytic cells; Label-free quantitative mass spectrometryen_US
dc.titleProteomics of tissue factor silencing in cardiomyocytic cells reveals a new role for this coagulation factor in splicing machinery controlen_US
dc.status.refereedYesen_US
dc.date.Accepted2015-01-25
dc.typeArticleen_US
dc.type.versionAccepted Manuscripten_US
refterms.dateFOA2018-07-25T13:55:50Z


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