Proteomics of tissue factor silencing in cardiomyocytic cells reveals a new role for this coagulation factor in splicing machinery control
dc.contributor.author | Lento, S. | * |
dc.contributor.author | Brioschi, M. | * |
dc.contributor.author | Barcella, S. | * |
dc.contributor.author | Nasim, Md. Talat | * |
dc.contributor.author | Ghilardi, S. | * |
dc.contributor.author | Barbieri, S.S. | * |
dc.contributor.author | Tremoli, E. | * |
dc.contributor.author | Banfi, C. | * |
dc.date.accessioned | 2016-03-23T09:48:45Z | |
dc.date.available | 2016-03-23T09:48:45Z | |
dc.date.issued | 2015-04-24 | |
dc.identifier.citation | Lento S, Brioschi M, Barcella S, Nasim MT et al (2015) Proteomics of tissue factor silencing in cardiomyocytic cells reveals a new role for this coagulation factor in splicing machinery control. Journal of Proteomics. 119: 75-89. | en_US |
dc.identifier.uri | http://hdl.handle.net/10454/8001 | |
dc.description | Yes | en_US |
dc.description.abstract | It has long been known that Tissue Factor (TF) plays a role in blood coagulation and has a direct thrombotic action that is closely related to cardiovascular risk, but it is becoming increasingly clear that it has a much wider range of biological functions that range from inflammation to immunity. It is also involved in maintaining heart haemostasis and structure, and the observation that it is down-regulated in the myocardium of patients with dilated cardiomyopathy suggests that it influences cell-to-cell contact stability and contractility, and thus contributes to cardiac dysfunction. However, the molecular mechanisms underlying these coagulation-independent functions have not yet been fully elucidated. In order to analyse the influence of TF on the cardiomyocitic proteome, we used functional biochemical approaches incorporating label-free quantitative proteomics and gene silencing, and found that this provided a powerful means of identifying a new role for TF in regulating splicing machinery together with the expression of several proteins of the spliceosome, and mRNA metabolism with a considerable impact on cell viability. | en_US |
dc.language.iso | en | en_US |
dc.relation.isreferencedby | http://dx.doi.org/10.1016/j.jprot.2015.01.021 | en_US |
dc.rights | © 2015 Elsevier B.V. Full-text reproduced in accordance with the publisher’s self-archiving policy. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | en_US |
dc.subject | Tissue factor; Proteomics; Spliceosome; Splicing; Cardiomyocytic cells; Label-free quantitative mass spectrometry | en_US |
dc.title | Proteomics of tissue factor silencing in cardiomyocytic cells reveals a new role for this coagulation factor in splicing machinery control | en_US |
dc.status.refereed | Yes | en_US |
dc.date.Accepted | 2015-01-25 | |
dc.type | Article | en_US |
dc.type.version | Accepted Manuscript | en_US |
refterms.dateFOA | 2018-07-25T13:55:50Z |