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dc.contributor.authorLeoni, G.*
dc.contributor.authorRattray, Marcus*
dc.contributor.authorFulton, D.*
dc.contributor.authorRivera, A.*
dc.contributor.authorButt, A.M.*
dc.date.accessioned2016-02-23T16:32:51Z
dc.date.available2016-02-23T16:32:51Z
dc.date.issued2014-02
dc.identifier.citationLeoni G, Rattray M, Fulton D et al (2014) Immunoablation of cells expressing the NG2 chondroitin sulphate proteoglycan. Journal of Anatomy. 224(4): 216-227.en_US
dc.identifier.urihttp://hdl.handle.net/10454/7810
dc.descriptionYesen_US
dc.description.abstractExpression of the transmembrane NG2 chondroitin sulphate proteoglycan (CSPG) defines a distinct population of NG2-glia. NG2-glia serve as a regenerative pool of oligodendrocyte progenitor cells in the adult central nervous system (CNS), which is important for demyelinating diseases such as multiple sclerosis, and are a major component of the glial scar that inhibits axon regeneration after CNS injury. In addition, NG2-glia form unique neuron–glial synapses with unresolved functions. However, to date it has proven difficult to study the importance of NG2-glia in any of these functions using conventional transgenic NG2 ‘knockout’ mice. To overcome this, we aimed to determine whether NG2-glia can be targeted using an immunotoxin approach. We demonstrate that incubation in primary anti-NG2 antibody in combination with secondary saporin-conjugated antibody selectively kills NG2-expressing cells in vitro. In addition, we provide evidence that the same protocol induces the loss of NG2-glia without affecting astrocyte or neuronal numbers in cerebellar brain slices from postnatal mice. This study shows that targeting the NG2 CSPG with immunotoxins is an effective and selective means for killing NG2-glia, which has important implications for studying the functions of these enigmatic cells both in the normal CNS, and in demyelination and degeneration.en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttp://doi.org/10.1111/joa.12141en_US
dc.rights© 2014 The Authors. Published Open Access by Wiley. Reproduced in accordance with the publisher's self-archiving policy.en_US
dc.subjectGlia; Immunoablation; Immunotoxin; NG2 proteoglycan; Oligodendrocyte progenitor cells; Oligodendrocyte precursor cellsen_US
dc.titleImmunoablation of cells expressing the NG2 chondroitin sulphate proteoglycanen_US
dc.status.refereedYesen_US
dc.type.versionPublished version
refterms.dateFOA2018-07-25T14:17:38Z


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