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dc.contributor.authorGrant, Colin A.*
dc.contributor.authorTwigg, Peter C.*
dc.contributor.authorSaeed, Rida F.*
dc.contributor.authorLawson, G.*
dc.contributor.authorFalconer, Robert A.*
dc.contributor.authorShnyder, Steven D.*
dc.date.accessioned2016-01-25T11:30:40Z
dc.date.available2016-01-25T11:30:40Z
dc.date.issued2016-03-01
dc.identifier.citationGrant CA, Twigg PC, Saeed RF, Lawson G, Falconer RA and Shnyder SD (2016) The Effect of Polysialic Acid Expression on Glioma Cell Nano-mechanics. BioNanoScience. 6(1): 81-84.en_US
dc.identifier.urihttp://hdl.handle.net/10454/7700
dc.descriptionYesen_US
dc.description.abstractPolysialic acid (PolySia) is an important carbohydrate bio-polymer that is commonly over-expressed on tumours of neuroendocrine origin and plays a key role in tumour progression. PolySia exclusively decorates the neural cell adhesion molecule (NCAM) on tumour cell membranes, modulating cell-cell interactions, motility and invasion. In this preliminary study, we examine the nano-mechanical properties of isogenic C6 rat glioma cells - transfected cells engineered to express the enzyme polysialyltransferase ST8SiaII, which synthesises polySia (C6-STX cells) and wild type cells (C6-WT). We demonstrate that polySia expression leads to reduced elastic and adhesive properties but also more visco-elastic compared to non-expressing wild type cells. Whilst differences in cell elasticity between healthy and cancer cells is regularly assigned to changes in the cytoskeleton, we show that in this model system the change in properties at the nano-level is due to the polySia on the transfected cell membrane surface.en_US
dc.language.isoenen_US
dc.rights© 2016 The Authors. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.en_US
dc.subjectAFM; Nano-mechanics; Cell elasticity; Tumour dissemination; Polysialic aciden_US
dc.titleThe Effect of Polysialic Acid Expression on Glioma Cell Nano-mechanicsen_US
dc.status.refereedYesen_US
dc.typeArticleen_US
dc.type.versionAccepted Manuscripten_US
refterms.dateFOA2018-07-25T14:16:41Z


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