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dc.contributor.authorTobin, Desmond J.*
dc.date.accessioned2015-10-26T16:21:38Z
dc.date.available2015-10-26T16:21:38Z
dc.date.issued2010-10
dc.identifier.citationTobin DJ (2011) Ex vivo organ culture of human hair follicles: a model epithelial-neuroectodermal-mesenchymal interaction system. Methods in Molecular Biology, Vol. 695:213-27.en_US
dc.identifier.urihttp://hdl.handle.net/10454/7452
dc.descriptionnoen_US
dc.description.abstractThe development of hair follicle organ culture techniques is a significant milestone in cutaneous biology research. The hair follicle, or more accurately the "pilo-sebaceous unit", encapsulates all the important physiologic processes found in the human body; controlled cell growth/death, interactions between cells of different histologic type, cell differentiation and migration, and hormone responsitivity to name a few. Thus, the value of the hair follicle as a model for biological scientific research goes way beyond its scope for cutaneous biology or dermatology alone. Indeed, the recent and dramatic upturn in interest in hair follicle biology has focused principally on the pursuit of two of biology's holy grails; post-embryonic morphogenesis and control of cyclical tissue activity. The hair follicle organ culture model, pioneered by Philpott and colleagues, ushered in an exceptionally accessible way to assess how cells of epithelial (e.g., keratinocytes), mesenchymal (e.g., fibroblasts), and neuroectodermal (e.g., melanocytes) origin interact in a three-dimensional manner. Moreover, this assay system allows us to assess how various natural and pharmacologic agents affect complex tissues for growth modulation. In this article, I focus on the culture of the human hair follicle mini-organ, discussing both the practical issues involved and some possible research applications of this assay.en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttp://dx.doi.org/10.1007%2F978-1-60761-984-0_14en_US
dc.subjectKeratinocytes; Fibroblasts; Melanocytes; Mesenchymal; Epithelial; Neuroectodermal; Neural crest; Stem cells; Human hair folliclesen_US
dc.titleEx vivo organ culture of human hair follicles: a model epithelial-neuroectodermal-mesenchymal interaction system.en_US
dc.status.refereedyesen_US
dc.typeArticleen_US
dc.type.versionNo full-text available in the repositoryen_US


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