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dc.contributor.authorBaris, O.R.*
dc.contributor.authorKlose, A.*
dc.contributor.authorKloepper, J.E.*
dc.contributor.authorWeiland, D.*
dc.contributor.authorNeuhaus, J.F.G.*
dc.contributor.authorSchauen, M.*
dc.contributor.authorWille, A.*
dc.contributor.authorMüller, A.*
dc.contributor.authorMerkwirth, C.*
dc.contributor.authorLanger, T.*
dc.contributor.authorLarsson, N-G.*
dc.contributor.authorKrieg, T.*
dc.contributor.authorTobin, Desmond J.*
dc.contributor.authorPaus, R.*
dc.contributor.authorWiesner, R.J.*
dc.date.accessioned2015-10-20T11:27:06Z
dc.date.available2015-10-20T11:27:06Z
dc.date.issued2011-09
dc.identifier.citationBaris OR, Klose A, Kloepper JE, Weiland D, Neuhaus JF, Schauen M, Wille A, Müller A, Merkwirth C, Langer T, Larsson NG, Krieg T, Tobin DJ, Paus R and Wiesner RJ (2011) The mitochondrial electron transport chain is dispensable for proliferation and differentiation of epidermal progenitor cells, Stem Cells 29 (9):1459-68.en_US
dc.identifier.urihttp://hdl.handle.net/10454/7444
dc.descriptionnoen_US
dc.description.abstractTissue stem cells and germ line or embryonic stem cells were shown to have reduced oxidative metabolism, which was proposed to be an adaptive mechanism to reduce damage accumulation caused by reactive oxygen species. However, an alternate explanation is that stem cells are less dependent on specialized cytoplasmic functions compared with differentiated cells, therefore, having a high nuclear-to-cytoplasmic volume ratio and consequently a low mitochondrial content. To determine whether stem cells rely or not on mitochondrial respiration, we selectively ablated the electron transport chain in the basal layer of the epidermis, which includes the epidermal progenitor/stem cells (EPSCs). This was achieved using a loxP-flanked mitochondrial transcription factor A (Tfam) allele in conjunction with a keratin 14 Cre transgene. The epidermis of these animals (TfamEKO) showed a profound depletion of mitochondrial DNA and complete absence of respiratory chain complexes. However, despite a short lifespan due to malnutrition, epidermal development and skin barrier function were not impaired. Differentiation of epidermal layers was normal and no proliferation defect or major increase of apoptosis could be observed. In contrast, mice with an epidermal ablation of prohibitin-2, a scaffold protein in the inner mitochondrial membrane, displayed a dramatic phenotype observable already in utero, with severely impaired skin architecture and barrier function, ultimately causing death from dehydration shortly after birth. In conclusion, we here provide unequivocal evidence that EPSCs, and probably tissue stem cells in general, are independent of the mitochondrial respiratory chain, but still require a functional dynamic mitochondrial compartment.en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttp://dx.doi.org/10.1002/stem.695en_US
dc.subjectREF 2014; Stem cells; Oxidative metabolism; Mitochondrial dynamics; Epidermal developmenten_US
dc.titleThe Mitochondrial Electron Transport Chain Is Dispensable for Proliferation and Differentiation of Epidermal Progenitor Cells.en_US
dc.status.refereedyesen_US
dc.typeArticleen_US
dc.type.versionNo full-text available in the repositoryen_US


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