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    Recombinant Lucilia Sericata chymotrypsin in a topical hydrogel formulation degrades human wound eschar ex vivo.

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    Publication date
    2011-06
    Author
    Britland, Stephen T.
    Smith, Annie G.
    Finter, Wayne
    Eagland, D.
    Vowden, Kath
    Vowden, Peter
    Telford, G.
    Brown, A.
    Pritchard, D.I.
    Keyword
    Biotherapy; Lucilia sericata; Chronic wound therapy; Debridement; Larval biotherapy; Topical hydrogel formulation
    Peer-Reviewed
    yes
    
    Metadata
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    Abstract
    Larval biotherapy is a debridement tool used in wound management. The mechanism of action involves degradation of eschar by serine proteases including chymotrypsin within the alimentary fluids of first instar Lucilia sericata. With the rationale of obviating some limitations of biotherapy, including cost, complexity of use, and patient reticence, the present study describes a mobile hydrogel formulation containing freeze-dried recombinant L. sericata chymotrypsin designed for topical application. Neither freeze-drying nor formulation into the hydrogel significantly attenuated the measured activity of released enzyme compared to fresh-frozen enzyme in aqueous solution. Gel electrophoresis confirmed qualitatively that the chymotrypsin/hydrogel formulation both with and without supplementary urea at 10% w/v degraded human chronic wound eschar ex vivo. Mindful that the hallmark of intractability of chronic wounds is aberrant biochemistry, the pH activity profile for the enzyme/hydrogel formulation was compared with exudate pH in chronic wounds of mixed aetiology in a cohort of 48 hospital in-patients. Five patients' wounds were acidic, however, the remainder were predominantly alkaline and coincided with the pH optimum for the insect enzyme. Thus, a recombinant L. sericata chymotrypsin and hydrogel formulation could represent a pragmatic alternative to larval therapy for the management of chronic wounds.
    URI
    http://hdl.handle.net/10454/7438
    Version
    No full-text available in the repository
    Citation
    Britland S, Smith AG, Finter W, Eagland D, Vowden K, Vowden P, Telford G, Brown A and Pritchard D (2011) Recombinant Lucilia sericata chymotrypsin in a topical hydrogel formulation degrades human wound eschar ex vivo. Biotechnology Progress, 27 (3): 870-4.
    Link to publisher’s version
    http://dx.doi.org/10.1002/btpr.587
    Type
    Article
    Collections
    Life Sciences Publications

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