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dc.contributor.authorMassey, Karen A.
dc.contributor.authorNicolaou, Anna
dc.date.accessioned2015-10-20T09:08:40Z
dc.date.available2015-10-20T09:08:40Z
dc.date.issued2013-06
dc.identifier.citationMassey K A and Nicolaou A (2013) Lipidomics of oxidized polyunsaturated fatty acids. Free Radical Biology and Medicine, 59: 45-55.en_US
dc.identifier.urihttp://hdl.handle.net/10454/7431
dc.descriptionnoen_US
dc.description.abstractLipid mediators are produced from the oxidation of polyunsaturated fatty acids through enzymatic and free radical-mediated reactions. When subject to oxygenation via cyclooxygenases, lipoxygenases, and cytochrome P450 monooxygenases, polyunsaturated fatty acids give rise to an array of metabolites including eicosanoids, docosanoids, and octadecanoids. These potent bioactive lipids are involved in many biochemical and signaling pathways, with inflammation being of particular importance. Moreover, because they are produced by more than one pathway and substrate, and are present in a variety of biological milieus, their analysis is not always possible with conventional assays. Liquid chromatography coupled to electrospray mass spectrometry offers a versatile and sensitive approach for the analysis of bioactive lipids, allowing specific and accurate quantitation of multiple species present in the same sample. Here we explain the principles of this approach to mediator lipidomics and present detailed protocols for the assay of enzymatically produced oxygenated metabolites of polyunsaturated fatty acids that can be tailored to answer biological questions or facilitate assessment of nutritional and pharmacological interventions.en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttp://dx.doi.org/10.1016/j.freeradbiomed.2012.08.565en_US
dc.subjectCyclooxygenase; Lipoxygenase; Cytochrome P450; Tandem mass spectrometry; Bioactive lipids; Eicosanoids; Docosanoids; Octadecanoids; Free radicals; Polyunsaturated fatty acid (PUFA) metabolitesen_US
dc.titleLipidomics of oxidized polyunsaturated fatty acids.en_US
dc.status.refereedyesen_US
dc.typeArticleen_US
dc.type.versionNo full-text available in the repositoryen_US


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