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dc.contributor.authorMassey, Karen A.*
dc.contributor.authorNicolaou, Anna*
dc.date.accessioned2015-10-20T09:08:40Z
dc.date.available2015-10-20T09:08:40Z
dc.date.issued2013-06
dc.identifier.citationMassey K A and Nicolaou A (2013) Lipidomics of oxidized polyunsaturated fatty acids. Free Radical Biology and Medicine, 59: 45-55.
dc.identifier.urihttp://hdl.handle.net/10454/7431
dc.descriptionNo
dc.description.abstractLipid mediators are produced from the oxidation of polyunsaturated fatty acids through enzymatic and free radical-mediated reactions. When subject to oxygenation via cyclooxygenases, lipoxygenases, and cytochrome P450 monooxygenases, polyunsaturated fatty acids give rise to an array of metabolites including eicosanoids, docosanoids, and octadecanoids. These potent bioactive lipids are involved in many biochemical and signaling pathways, with inflammation being of particular importance. Moreover, because they are produced by more than one pathway and substrate, and are present in a variety of biological milieus, their analysis is not always possible with conventional assays. Liquid chromatography coupled to electrospray mass spectrometry offers a versatile and sensitive approach for the analysis of bioactive lipids, allowing specific and accurate quantitation of multiple species present in the same sample. Here we explain the principles of this approach to mediator lipidomics and present detailed protocols for the assay of enzymatically produced oxygenated metabolites of polyunsaturated fatty acids that can be tailored to answer biological questions or facilitate assessment of nutritional and pharmacological interventions.
dc.language.isoen
dc.subjectCyclooxygenase
dc.subjectLipoxygenase
dc.subjectCytochrome P450
dc.subjectTandem mass spectrometry
dc.subjectBioactive lipids
dc.subjectEicosanoids
dc.subjectDocosanoids
dc.subjectOctadecanoids
dc.subjectFree radicals
dc.subjectPolyunsaturated fatty acid (PUFA) metabolites
dc.titleLipidomics of oxidized polyunsaturated fatty acids.
dc.status.refereedYes
dc.typeArticle
dc.type.versionNo full-text in the repository
dc.identifier.doihttps://doi.org/10.1016/j.freeradbiomed.2012.08.565
dc.openaccess.statusclosedAccess


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