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dc.contributor.authorEvans, K.*
dc.contributor.authorBhamra, I.*
dc.contributor.authorWheelhouse, Richard T.*
dc.contributor.authorArnold, J.R.P.*
dc.contributor.authorCosstick, R.*
dc.contributor.authorFisher, J.*
dc.date.accessioned2015-06-05T07:54:51Z
dc.date.available2015-06-05T07:54:51Z
dc.date.issued2015
dc.identifier.citationEvans K, Bhambra I, Wheelhouse RT et al (2015) Stabilization of a bimolecular triplex by 3’-S-phosphorothiolate modifications: an NMR and UV thermal melting investigation. Chemistry: A European Journal. 21(19): 7278-7284.en_US
dc.identifier.urihttp://hdl.handle.net/10454/7229
dc.descriptionYesen_US
dc.description.abstractTriplexes formed from oligonucleic acids are key to a number of biological processes. They have attracted attention as molecular biology tools and as a result of their relevance in novel therapeutic strategies. The recognition properties of single-stranded nucleic acids are also relevant in third-strand binding. Thus, there has been considerable activity in generating such moieties, referred to as triplex forming oligonucleotides (TFOs). Triplexes, composed of Watson–Crick (W–C) base-paired DNA duplexes and a Hoogsteen base-paired RNA strand, are reported to be more thermodynamically stable than those in which the third strand is DNA. Consequently, synthetic efforts have been focused on developing TFOs with RNA-like structural properties. Here, the structural and stability studies of such a TFO, composed of deoxynucleic acids, but with 3′-S-phosphorothiolate (3′-SP) linkages at two sites is described. The modification results in an increase in triplex melting temperature as determined by UV absorption measurements. 1H NMR analysis and structure generation for the (hairpin) duplex component and the native and modified triplexes revealed that the double helix is not significantly altered by the major groove binding of either TFO. However, the triplex involving the 3′-SP modifications is more compact. The 3′-SP modification was previously shown to stabilise G-quadruplex and i-motif structures and therefore is now proposed as a generic solution to stabilising multi-stranded DNA structures.en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttp://dx.doi.org/10.1002/chem.201500369en_US
dc.rights© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Full-text reproduced in accordance with the publisher's self-archiving policy.en_US
dc.subject3'-S-phosphorothiolate; DNA structures; NMR spectroscopy; Nucleic acids; UV thermal meltingen_US
dc.titleStabilization of a Bimolecular Triplex by 3′-S-Phosphorothiolate Modifications: An NMR and UV Thermal Melting Investigationen_US
dc.status.refereedYesen_US
dc.typeArticleen_US
dc.date.EndofEmbargo2016-03-21
dc.type.versionAccepted Manuscripten_US
refterms.dateFOA2018-07-25T11:29:13Z


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