BRADFORD SCHOLARS

    • Sign in
    View Item 
    •   Bradford Scholars
    • Life Sciences
    • Life Sciences Publications
    • View Item
    •   Bradford Scholars
    • Life Sciences
    • Life Sciences Publications
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of Bradford ScholarsCommunitiesAuthorsTitlesSubjectsPublication DateThis CollectionAuthorsTitlesSubjectsPublication Date

    My Account

    Sign in

    HELP

    Bradford Scholars FAQsCopyright Fact SheetPolicies Fact SheetDeposit Terms and ConditionsDigital Preservation Policy

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Stabilization of a Bimolecular Triplex by 3′-S-Phosphorothiolate Modifications: An NMR and UV Thermal Melting Investigation

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    View/Open
    wheelhouse_stabilization_of_a_bimolecular_triplex_chemistry_a_european_journal.pdf (500.7Kb)
    Download
    Publication date
    2015
    End of Embargo
    2016-03-21
    Author
    Evans, K.
    Bhamra, I.
    Wheelhouse, Richard T.
    Arnold, J.R.P.
    Cosstick, R.
    Fisher, J.
    Keyword
    3'-S-phosphorothiolate; DNA structures; NMR spectroscopy; Nucleic acids; UV thermal melting
    Rights
    © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Full-text reproduced in accordance with the publisher's self-archiving policy.
    Peer-Reviewed
    Yes
    
    Metadata
    Show full item record
    Abstract
    Triplexes formed from oligonucleic acids are key to a number of biological processes. They have attracted attention as molecular biology tools and as a result of their relevance in novel therapeutic strategies. The recognition properties of single-stranded nucleic acids are also relevant in third-strand binding. Thus, there has been considerable activity in generating such moieties, referred to as triplex forming oligonucleotides (TFOs). Triplexes, composed of Watson–Crick (W–C) base-paired DNA duplexes and a Hoogsteen base-paired RNA strand, are reported to be more thermodynamically stable than those in which the third strand is DNA. Consequently, synthetic efforts have been focused on developing TFOs with RNA-like structural properties. Here, the structural and stability studies of such a TFO, composed of deoxynucleic acids, but with 3′-S-phosphorothiolate (3′-SP) linkages at two sites is described. The modification results in an increase in triplex melting temperature as determined by UV absorption measurements. 1H NMR analysis and structure generation for the (hairpin) duplex component and the native and modified triplexes revealed that the double helix is not significantly altered by the major groove binding of either TFO. However, the triplex involving the 3′-SP modifications is more compact. The 3′-SP modification was previously shown to stabilise G-quadruplex and i-motif structures and therefore is now proposed as a generic solution to stabilising multi-stranded DNA structures.
    URI
    http://hdl.handle.net/10454/7229
    Version
    Accepted Manuscript
    Citation
    Evans K, Bhambra I, Wheelhouse RT et al (2015) Stabilization of a bimolecular triplex by 3’-S-phosphorothiolate modifications: an NMR and UV thermal melting investigation. Chemistry: A European Journal. 21(19): 7278-7284.
    Link to publisher’s version
    http://dx.doi.org/10.1002/chem.201500369
    Type
    Article
    Collections
    Life Sciences Publications

    entitlement

     
    DSpace software (copyright © 2002 - 2022)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.