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dc.contributor.authorBatman, Philip A.*
dc.contributor.authorKotler, D.P.*
dc.contributor.authorKapembwa, M.S.*
dc.contributor.authorBooth, D.*
dc.contributor.authorOrenstein, J.M.*
dc.contributor.authorScally, Andy J.*
dc.contributor.authorGriffin, G.*
dc.contributor.authorPotten, C.S.*
dc.date.accessioned2014-07-18T16:31:03Z
dc.date.available2014-07-18T16:31:03Z
dc.date.issued2007
dc.identifier.citationBatman, P. A., Kotler, D. P., Kapembwa, M. S., Booth, D., Potten, C. S., Orenstein, J. M., Scally, A. J., and Griffin, G. E. (2007) HIV enteropathy: crypt stem and transit cell hyperproliferation induces villous atrophy in HIV/Microsporidia-infected jejunal mucosa. AIDS, 21 (4): 433-9.en_US
dc.identifier.urihttp://hdl.handle.net/10454/6407
dc.description.abstractObjectives: The study aim was to analyse the kinetics of stem and transit cells in the crypts of jejunal mucosa infected with HIV and Microsporidia. Design: The size of villi, depth of crypts and proliferative activity of transit and stem cells in jejunal mucosa were measured using morphometric techniques. Methods: The surface area/volume ratio (S/V) of jejunal biopsies was estimated under light microscopy using a Weibel graticule. Crypt length was measured by counting enterocytes along the crypt side from the base to the villus junction, and the mean crypt length was calculated. The S/V and crypt lengths of the jejunal mucosa of 21 HIV and Microsporidia-infected test cases were compared with 14 control cases. The labelling index in relation to the crypt cell position of 10 of the test cases was analysed compared with 13 control cases. Results: Differences were found in the S/V and crypt length, and there was a negative correlation between S/V and crypt length in test and control cases combined. Cell labelling indices fell into low and high proliferation groups. There were significant differences in labelling indices between low proliferation test cases and controls, between high proliferation test cases and controls, and between high and low proliferation test cases. Conclusion: Villous atrophy induced by HIV and Microsporidia is attributed to crypt cell hyperplasia and the encroachment of crypt cells onto villi. These infections induce crypt hypertrophy by stimulating cell mitosis predominantly in transit cells but also in stem cells. Increased stem cell proliferation occurs only in high proliferation cases.en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttp://dx.doi.org/10.1097/QAD.0b013e3280142ee8en_US
dc.subjectStem cell kineticsen_US
dc.subjectTransit cell kineticsen_US
dc.subjectHyperproliferationen_US
dc.subjectHIV enteropathyen_US
dc.subjectVillous atrophyen_US
dc.subjectMicrosporidiaen_US
dc.subjectJejunal mucosaen_US
dc.subjectMicrosporidia infectionen_US
dc.subjectHIV infectionen_US
dc.titleHIV enteropathy: crypt stem and transit cell hyperproliferation induces villous atrophy in HIV/Microsporidia-infected jejunal mucosa.en_US
dc.status.refereedyesen_US
dc.typeArticleen_US
dc.type.versionpublished version paperen_US


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