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dc.contributor.advisorWright, Colin W.
dc.contributor.authorOkpako, Larry Commander*
dc.date.accessioned2014-05-02T16:32:28Z
dc.date.available2014-05-02T16:32:28Z
dc.date.issued2014-05-02
dc.identifier.urihttp://hdl.handle.net/10454/6304
dc.description.abstractMalaria affects hundreds of millions of people worldwide and equally claims hundreds of thousands of lives each year. With the current spread of drug resistance to standard antimalarial drugs like chloroquine and the emergence of artemisinin-resistant parasites, new antimalarial drugs and formulations are urgently needed. An ethnobotanical survey was carried out in this study in search of novel compounds with promising antiplasmodial activity. Using the ethnobotanical approach, a total of 61 plant species from 59 genera distributed in 34 plant families were found to be used traditionally for the treatment of malaria in Nigeria. Biological evaluation of the plant¿s methanolic extracts was assessed using the parasite lactate dehydrogenase (pLDH) assay against the chloroquine-sensitive (3D7) and chloroquine-resistant (K1) strains of Plasmodium falciparum. A total of five (5) plant species showed more potent antiplasmodial activities against the malaria parasites. These are Acanthospermum hispidum, Cassia occidentalis, Kaempferia aethiopica Prosopis africana and Physalis angulata with MIC values ranging between 7.815µg/ml to 31.25µg/ml (3D7 strain) and 15.63µg/ml to 62.50µg/ml (K1 strain) against the malaria parasites, respectively. Two plants, Prosopis africana (Leguminosae-mimosoideae) and Physalis angulata (Solanaceae) were selected for further study. The phytochemical investigation of the active chloroform extracts of P. africana and P. angulata yielded several compounds with three known alkaloids, namely, prosopinine (I), prosopine (II) and acetamide (III). Their structures were confirmed by MS, 1D and 2D NMR spectroscopy. Compounds I, II and III have moderate in vitro antiplasmodial activity against the malaria parasites. Both chloroquine and artemether were used as standard control.en_US
dc.description.sponsorshipAssociation of Commonwealth Universities and the Commonwealth Scholarship Commission in the UK (Commonwealth Scholarship Reference Number: NGCS-2005-259).en_US
dc.language.isoenen_US
dc.rights<a rel="license" href="http://creativecommons.org/licenses/by-nc-nd/3.0/"><img alt="Creative Commons License" style="border-width:0" src="http://i.creativecommons.org/l/by-nc-nd/3.0/88x31.png" /></a><br />The University of Bradford theses are licenced under a <a rel="license" href="http://creativecommons.org/licenses/by-nc-nd/3.0/">Creative Commons Licence</a>.eng
dc.subjectMalariaen_US
dc.subjectEthnobotanical surveyen_US
dc.subjectMedicinal plantsen_US
dc.subjectAlkaloidsen_US
dc.subjectPlasmodium falciparumen_US
dc.subjectDrug resistanceen_US
dc.subjectAntiplasmodial activityen_US
dc.subjectPhysalis angulataen_US
dc.subjectProsopis africanaen_US
dc.titleInvestigation of selected Nigerian medicinal plants as a source of new antimalarial agents. Isolation of phytochemicals from some Nigerian medicinal plants using chromatographic techniques and their evaluation for antiplasmodial activity.en_US
dc.type.qualificationlevelresearch mastersen_US
dc.publisher.institutionUniversity of Bradfordeng
dc.publisher.departmentBradford School of Pharmacyen_US
dc.typeThesiseng
dc.type.qualificationnameMPhilen_US
dc.date.awarded2012
refterms.dateFOA2018-07-19T12:43:12Z


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