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dc.contributor.authorShakibaei, M.*
dc.contributor.authorCsaki, C.*
dc.contributor.authorNebrich, S.*
dc.contributor.authorMobasheri, A.*
dc.date.accessioned2014-04-28T11:20:02Z
dc.date.available2014-04-28T11:20:02Z
dc.date.issued2008
dc.identifier.citationShakibaei, M., Csaki, C., Nebrich, S., Mobasheri, A. (2008) Resveratrol suppresses interleukin-1beta-induced inflammatory signaling and apoptosis in human articular chondrocytes: potential for use as a novel nutraceutical for the treatment of osteoarthritis. Biochem Pharmacology, 76 (11), 1426-1439.
dc.identifier.urihttp://hdl.handle.net/10454/6181
dc.descriptionNo
dc.description.abstractOsteoarthritis is an inflammatory disease of load-bearing synovial joints that is currently treated with drugs that exhibit numerous side effects and are only temporarily effective on pain, the main symptom of the disease. Consequently, there is an acute need for novel, safe and more effective chemotherapeutic agents for the treatment of osteoarthritis and related arthritic diseases. Resveratrol is a phytoalexin stilbene produced naturally by plants including red grapes, peanuts and various berries. Recent research in various cell models has demonstrated that resveratrol is safe and has potent anti-inflammatory properties. However, its potential for treating arthritic conditions has not been explored. In this study we provide experimental evidence that resveratrol inhibits the expression of VEGF, MMP-3, MMP-9 and COX-2 in human articular chondrocytes stimulated with the pro-inflammatory cytokine IL-1beta. Since these gene products are regulated by the transcription factor NF-kappaB, we investigated the effects of resveratrol on IL-1beta-induced NF-kappaB signaling pathway. Resveratrol, like N-Ac-Leu-Leu-norleucinal (ALLN) suppressed IL-1beta-induced proteasome function and the degradation of IkappaBalpha (an inhibitor of NF-kappaB) without affecting IkappaBalpha kinase activation, IkappaBalpha-phosphorylation or IkappaBalpha-ubiquitination which suppressed nuclear translocation of the p65 subunit of NF-kappaB and its phosphorylation. Furthermore, we observed that resveratrol as well as ALLN inhibited IL-1beta-induced apoptosis, caspase-3 activation and PARP cleavage in human articular chondrocytes. In summary, our results suggest that resveratrol suppresses apoptosis and inflammatory signaling through its actions on the NF-kappaB pathway in human chondrocytes. We propose that resveratrol should be explored further for the prophylactic treatment of osteoarthritis in humans and companion animals.
dc.language.isoen
dc.subjectApoptosis
dc.subjectDrug effects
dc.subjectPhysiology
dc.subjectBlotting
dc.subjectWestern
dc.subjectCartilage
dc.subjectArticular
dc.subjectCytology
dc.subjectMetabolism
dc.subjectCell nucleus
dc.subjectCells
dc.subjectCultured
dc.subjectChondrocytes
dc.subjectUltrastructure
dc.subjectDietary supplements
dc.subjectDose-response relationship
dc.subjectExtracellular matrix
dc.subjectHumans
dc.subjectInflammation mediators
dc.subjectInterleukin-1beta
dc.subjectMicroscopy
dc.subjectElectron
dc.subjectTransmission
dc.subjectNF-kappa B
dc.subjectOsteoarthritis
dc.subjectResveratrol
dc.subjectProtein transport
dc.subjectSignal transduction
dc.subjectStilbenes
dc.subjectREF 2014
dc.titleResveratrol suppresses interleukin-1beta-induced inflammatory signaling and apoptosis in human articular chondrocytes: potential for use as a novel nutraceutical for the treatment of osteoarthritis
dc.typeArticle
dc.type.versionNo full-text in the repository
dc.identifier.doihttps://doi.org/10.1016/j.bcp.2008.05.029
dc.openaccess.statusclosedAccess


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