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dc.contributor.authorLeung, Man Ching*
dc.contributor.authorSutton, Chris W.*
dc.contributor.authorFenton, D.A.*
dc.contributor.authorTobin, Desmond J.*
dc.date.accessioned2014-04-28T10:52:21Z
dc.date.available2014-04-28T10:52:21Z
dc.date.issued2010
dc.identifier.citationLeung, M. C., Sutton, C. W., Fenton, D. A., Tobin, D. J. (2010) Trichohyalin is a potential major autoantigen in human alopecia areata. J Proteome Res, 9 (10), 5153-63.
dc.identifier.urihttp://hdl.handle.net/10454/6066
dc.description.abstractSeveral lines of evidence support an autoimmune basis for alopecia areata (AA), a common putative autoimmune hair loss disorder. However, definitive support is lacking largely because the identity of hair follicle (HF) autoantigen(s) involved in its pathogenesis remains unknown. Here, we isolated AA-reactive HF-specific antigens from normal human scalp anagen HF extracts by immunoprecipitation using serum antibodies from 10 AA patients. Samples were analyzed by LC-MALDI-TOF/TOF mass spectrometry, which indicated strong reactivity to the hair growth phase-specific structural protein trichohyalin in all AA sera. Keratin 16 (K16) was also identified as another potential AA-relevant target HF antigen. Double immunofluorescence studies using AA (and control sera) together with a monoclonal antibody to trichohyalin revealed that AA sera contained immunoreactivity that colocalized with trichohyalin in the growth phase-specific inner root sheath of HF. Furthermore, a partial colocalization of AA serum reactivity with anti-K16 antibody was observed in the outer root sheath of the HF. In summary, this study supports the involvement of an immune response to anagen-specific HFs antigens in AA and specifically suggests that an immune response to trichohyalin and K16 may have a role in the pathogenesis of the enigmatic disorder.
dc.relation.isreferencedbyhttp://dx.doi.org/10.1021/pr100422u
dc.subjectAdult
dc.subjectAlopecia Areata; Blood; Immunology
dc.subjectAutoantigens; Analysis
dc.subjectFemale
dc.subjectFluorescent antibody technique; Indirect
dc.subjectHair follicle; Pathology
dc.subjectHumans
dc.subjectIntermediate filament proteins
dc.subjectKeratin-16
dc.subjectMale
dc.subjectProtein precursors
dc.subjectSpectrometry; Mass; Matrix-assisted laser desorption-Ionization
dc.subjectREF 2014
dc.titleTrichohyalin is a potential major autoantigen in human alopecia areata
dc.typeArticle


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