Trichohyalin is a potential major autoantigen in human alopecia areata
dc.contributor.author | Leung, Man Ching | * |
dc.contributor.author | Sutton, Chris W. | * |
dc.contributor.author | Fenton, D.A. | * |
dc.contributor.author | Tobin, Desmond J. | * |
dc.date.accessioned | 2014-04-28T10:52:21Z | |
dc.date.available | 2014-04-28T10:52:21Z | |
dc.date.issued | 2010 | |
dc.identifier.citation | Leung, M. C., Sutton, C. W., Fenton, D. A., Tobin, D. J. (2010) Trichohyalin is a potential major autoantigen in human alopecia areata. J Proteome Res, 9 (10), 5153-63. | |
dc.identifier.uri | http://hdl.handle.net/10454/6066 | |
dc.description.abstract | Several lines of evidence support an autoimmune basis for alopecia areata (AA), a common putative autoimmune hair loss disorder. However, definitive support is lacking largely because the identity of hair follicle (HF) autoantigen(s) involved in its pathogenesis remains unknown. Here, we isolated AA-reactive HF-specific antigens from normal human scalp anagen HF extracts by immunoprecipitation using serum antibodies from 10 AA patients. Samples were analyzed by LC-MALDI-TOF/TOF mass spectrometry, which indicated strong reactivity to the hair growth phase-specific structural protein trichohyalin in all AA sera. Keratin 16 (K16) was also identified as another potential AA-relevant target HF antigen. Double immunofluorescence studies using AA (and control sera) together with a monoclonal antibody to trichohyalin revealed that AA sera contained immunoreactivity that colocalized with trichohyalin in the growth phase-specific inner root sheath of HF. Furthermore, a partial colocalization of AA serum reactivity with anti-K16 antibody was observed in the outer root sheath of the HF. In summary, this study supports the involvement of an immune response to anagen-specific HFs antigens in AA and specifically suggests that an immune response to trichohyalin and K16 may have a role in the pathogenesis of the enigmatic disorder. | |
dc.subject | Adult | |
dc.subject | Alopecia Areata; Blood; Immunology | |
dc.subject | Autoantigens; Analysis | |
dc.subject | Female | |
dc.subject | Fluorescent antibody technique; Indirect | |
dc.subject | Hair follicle; Pathology | |
dc.subject | Humans | |
dc.subject | Intermediate filament proteins | |
dc.subject | Keratin-16 | |
dc.subject | Male | |
dc.subject | Protein precursors | |
dc.subject | Spectrometry; Mass; Matrix-assisted laser desorption-Ionization | |
dc.subject | REF 2014 | |
dc.title | Trichohyalin is a potential major autoantigen in human alopecia areata | |
dc.type | Article | |
dc.identifier.doi | https://doi.org/10.1021/pr100422u |