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dc.contributor.authorLaubenthal, Julian
dc.contributor.authorZlobinskaya, O.
dc.contributor.authorPoterlowicz, Krzysztof
dc.contributor.authorBaumgartner, Adolf
dc.contributor.authorGdula, Michal R.
dc.contributor.authorFthenou, E.
dc.contributor.authorKeramarou, M.
dc.contributor.authorHepworth, S.J.
dc.contributor.authorKleinjans, J.C.
dc.contributor.authorvan Schooten, F.J.
dc.contributor.authorBrunborg, G.
dc.contributor.authorGodschalk, R.W.
dc.contributor.authorSchmid, Thomas E.
dc.contributor.authorAnderson, Diana
dc.date.accessioned2014-04-28T10:52:21Z
dc.date.available2014-04-28T10:52:21Z
dc.date.issued2012
dc.identifier.citationLaubenthal, J., Zlobinskaya, O., Poterlowicz, K., Baumgartner, A., Gdula, M. R., Fthenou, E., Keramarou, M., Hepworth, S. J., Kleinjans, J. C., van Schooten, F. J., Brunborg, G., Godschalk, R. W., Schmid, T. E., Anderson, D. (2012) Cigarette smoke-induced transgenerational alterations in genome stability in cord blood of human F1 offspring. FASEB J, 26 (10), 3946-56.
dc.identifier.urihttp://hdl.handle.net/10454/6063
dc.description.abstractThe relevance of preconceptional and prenatal toxicant exposures for genomic stability in offspring is difficult to analyze in human populations, because gestational exposures usually cannot be separated from preconceptional exposures. To analyze the roles of exposures during gestation and conception on genomic stability in the offspring, stability was assessed via the Comet assay and highly sensitive, semiautomated confocal laser scans of gammaH2AX foci in cord, maternal, and paternal blood as well as spermatozoa from 39 families in Crete, Greece, and the United Kingdom. With use of multivariate linear regression analysis with backward selection, preconceptional paternal smoking (% tail DNA: P>0.032; gammaH2AX foci: P>0.018) and gestational maternal (% tail DNA: P>0.033) smoking were found to statistically significantly predict DNA damage in the cord blood of F1 offspring. Maternal passive smoke exposure was not identified as a predictor of DNA damage in cord blood, indicating that the effect of paternal smoking may be transmitted via the spermatozoal genome. Taken together, these studies reveal a role for cigarette smoke in the induction of DNA alterations in human F1 offspring via exposures of the fetus in utero or the paternal germline. Moreover, the identification of transgenerational DNA alterations in the unexposed F1 offspring of smoking-exposed fathers supports the claim that cigarette smoke is a human germ cell mutagen.
dc.relation.isreferencedbyhttp://dx.doi.org/10.1096/fj.11-201194
dc.subjectAdolescent
dc.subjectAdult
dc.subjectComet Assay
dc.subjectCotinine; Diagnostic use; Urine
dc.subjectDNA Damage; Drug effects; Genetics
dc.subjectFemale
dc.subjectFetal Blood; Metabolism
dc.subjectGenomic Instability
dc.subjectHumans
dc.subjectInfant; Newborn
dc.subjectMale
dc.subjectMaternal Exposure
dc.subjectMultivariate Analysis
dc.subjectPregnancy
dc.subjectSmoking
dc.subjectYoung Adult
dc.subjectREF 2014
dc.titleCigarette smoke-induced transgenerational alterations in genome stability in cord blood of human F1 offspring
dc.typeArticle


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