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    An interaction between KSHV ORF57 and UIF provides mRNA-adaptor redundancy in herpesvirus intronless mRNA export

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    Publication date
    2011
    Author
    Jackson, B.R.
    Boyne, James R.
    Noerenberg, M.
    Taylor, A.
    Hautbergue, G.M.
    Walsh, M.J.
    Wheat, R.
    Blackbourn, D.J.
    Wilson, S.A.
    Whitehouse, A.
    Keyword
    Active transport; Cell nucleus; Genetics
    Metabolism; Virology
    HEK293 Cells
    Herpesvirus 8
    Humans
    Nuclear proteins
    RNA; Messenger
    Viral
    RNA-binding
    Viral proteins
    REF 2014
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    Abstract
    The hTREX complex mediates cellular bulk mRNA nuclear export by recruiting the nuclear export factor, TAP, via a direct interaction with the export adaptor, Aly. Intriguingly however, depletion of Aly only leads to a modest reduction in cellular mRNA nuclear export, suggesting the existence of additional mRNA nuclear export adaptor proteins. In order to efficiently export Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs from the nucleus, the KSHV ORF57 protein recruits hTREX onto viral intronless mRNAs allowing access to the TAP-mediated export pathway. Similarly however, depletion of Aly only leads to a modest reduction in the nuclear export of KSHV intronless mRNAs. Herein, we identify a novel interaction between ORF57 and the cellular protein, UIF. We provide the first evidence that the ORF57-UIF interaction enables the recruitment of hTREX and TAP to KSHV intronless mRNAs in Aly-depleted cells. Strikingly, depletion of both Aly and UIF inhibits the formation of an ORF57-mediated nuclear export competent ribonucleoprotein particle and consequently prevents ORF57-mediated mRNA nuclear export and KSHV protein production. Importantly, these findings highlight that redundancy exists in the eukaryotic system for certain hTREX components involved in the mRNA nuclear export of intronless KSHV mRNAs.
    URI
    http://hdl.handle.net/10454/6028
    Citation
    Jackson, B. R., Boyne, J. R., Noerenberg, M., Taylor, A., Hautbergue, G. M., Walsh, M. J., Wheat, R., Blackbourn, D. J., Wilson, S. A., Whitehouse, A. (2011) An interaction between KSHV ORF57 and UIF provides mRNA-adaptor redundancy in herpesvirus intronless mRNA export. PLoS Pathogens, 7(7), e1002138.
    Link to publisher’s version
    http://dx.doi.org/10.1371/journal.ppat.1002138
    Type
    Article
    Collections
    Life Sciences Publications

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