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dc.contributor.authorFessing, Michael Y.*
dc.contributor.authorMardaryev, Andrei N.*
dc.contributor.authorGdula, Michal R.*
dc.contributor.authorSharov, A.A.*
dc.contributor.authorSharova, T.Y.*
dc.contributor.authorRapisarda, Valentina*
dc.contributor.authorGordon, K.B.*
dc.contributor.authorSmorodchenko, A.D.*
dc.contributor.authorPoterlowicz, Krzysztof*
dc.contributor.authorFerone, G.*
dc.contributor.authorKohwi, Y.*
dc.contributor.authorMissero, C.*
dc.contributor.authorKohwi-Shigematsu, T.*
dc.contributor.authorBotchkarev, Vladimir A.*
dc.date.accessioned2014-04-25T15:10:21Z
dc.date.available2014-04-25T15:10:21Z
dc.date.issued2011
dc.identifier.citationFessing MY, Mardaryev AN, Gdula MR et al. (2011) p63 regulates Satb1 to control tissue-specific chromatin remodeling during development of the epidermis. Journal of Cell Biology. 194(6): 825-839.
dc.identifier.urihttp://hdl.handle.net/10454/5968
dc.descriptionNo
dc.description.abstractDuring development, multipotent progenitor cells establish tissue-specific programs of gene expression. In this paper, we show that p63 transcription factor, a master regulator of epidermal morphogenesis, executes its function in part by directly regulating expression of the genome organizer Satb1 in progenitor cells. p63 binds to a proximal regulatory region of the Satb1 gene, and p63 ablation results in marked reduction in the Satb1 expression levels in the epidermis. Satb1(-/-) mice show impaired epidermal morphology. In Satb1-null epidermis, chromatin architecture of the epidermal differentiation complex locus containing genes associated with epidermal differentiation is altered primarily at its central domain, where Satb1 binding was confirmed by chromatin immunoprecipitation-on-chip analysis. Furthermore, genes within this domain fail to be properly activated upon terminal differentiation. Satb1 expression in p63(+/-) skin explants treated with p63 small interfering ribonucleic acid partially restored the epidermal phenotype of p63-deficient mice. These data provide a novel mechanism by which Satb1, a direct downstream target of p63, contributes in epidermal morphogenesis via establishing tissue-specific chromatin organization and gene expression in epidermal progenitor cells.
dc.relation.isreferencedbyhttp://dx.doi.org/10.1083/jcb.201101148
dc.subjectAnimals
dc.subject; Cell differentiation
dc.subject; Chromatin; Metabolism
dc.subject; Chromatin assembly and disassembly; Genetics
dc.subject; Epidermis; Cytology; Embryology
dc.subject; Gene expression regulation; Developmental
dc.subject; Genome
dc.subject; In situ hybridization; Fluorescence
dc.subject; Matrix attachment region binding proteins
dc.subject; Mice
dc.subject; Inbred C57BL
dc.subject; Phosphoproteins
dc.subject; Trans-activators
dc.subject; REF 2014
dc.titlep63 regulates Satb1 to control tissue-specific chromatin remodeling during development of the epidermis
dc.status.refereedYes
dc.typeArticle
dc.type.versionNo full-text in the repository


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