Show simple item record

dc.contributor.authorCorrêa, Sonia A.L.*
dc.contributor.authorMuller, Jurgen*
dc.contributor.authorCollingridge, G.L.*
dc.contributor.authorMarrion, N.V.*
dc.date.accessioned2014-04-25T14:41:22Z
dc.date.available2014-04-25T14:41:22Z
dc.date.issued2009
dc.identifier.citationCorrea SA, Muller J, Collingridge GL and Marrion NV (2009) Rapid endocytosis provides restricted somatic expression of a K+ channel in central neurons. Journal of Cell Science. 122(Pt 22): 4186-4194.
dc.identifier.urihttp://hdl.handle.net/10454/5941
dc.descriptionNo
dc.description.abstractTrafficking motifs present in the intracellular regions of ion channels affect their subcellular location within neurons. The mechanisms that control trafficking to dendrites of central neurons have been identified, but it is not fully understood how channels are localized to the soma. We have now identified a motif within the calcium-activated potassium channel K(Ca)2.1 (SK1) that results in somatic localization. Transfection of hippocampal neurons with K(Ca)2.1 subunits causes expression of functional channels in only the soma and proximal processes. By contrast, expressed K(Ca)2.3 subunits are located throughout the processes of transfected neurons. Point mutation of K(Ca)2.1 within this novel motif to mimic a sequence present in the C-terminus of K(Ca)2.3 causes expression of K(Ca)2.1 subunits throughout the processes. We also demonstrate that blocking of clathrin-mediated endocytosis causes K(Ca)2.1 subunit expression to mimic that of the mutated subunit. The role of this novel motif is therefore not to directly target trafficking of the channel to subcellular compartments, but to regulate channel location by subjecting it to rapid clathrin-mediated endocytosis.
dc.subjectAmino acid motifs
dc.subject; Animals
dc.subject; Cells
dc.subject; Clathrin
dc.subject; Endocytosis
dc.subject; Hippocampus
dc.subject; Neurons
dc.subject; Point mutation
dc.subject; Protein subunits
dc.subject; Protein transport
dc.subject; Rats
dc.subject; Small-conductance calcium-activated potassium channels
dc.subject; REF 2014
dc.titleRapid endocytosis provides restricted somatic expression of a K+ channel in central neurons
dc.status.refereedYes
dc.typeArticle
dc.type.versionNo full-text in the repository
dc.identifier.doihttps://doi.org/10.1242/jcs.058420


This item appears in the following Collection(s)

Show simple item record