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dc.contributor.advisorMarshall, Kay M.
dc.contributor.authorSabar, Uzmah Jabeen
dc.date.accessioned2013-11-21T15:44:26Z
dc.date.available2013-11-21T15:44:26Z
dc.date.issued2013-11-21
dc.identifier.urihttp://hdl.handle.net/10454/5691
dc.description.abstractProstaglandins are integral mediators in reproductive processes but their exact role in uterine function is still not clear. In addition, ethical restraints have limited the availability of human tissue to investigate uterine prostanoid receptor populations. The aim of this thesis was to characterise the prostanoid receptors on the human and rat myometrium in order to evaluate the potential of the rat as an animal model of human uterine function and disease. For functional analysis of myometrial prostanoid receptors the immersion technique was utilised. LC-ESI-MS/MS was also used to measure the ex vivo myometrial release of prostanoid metabolites. The results show that both the rat and human uterus displays cyclical changes in uterine motility, with myogenicity greatest in the follicular and oestrus stages. The data also indicate that whilst the human uterus is responsive to EP3, EP2, TP, FP and IP receptor agonists, a functional population of only EP3, EP2 and FP receptors is present on the rat uterus, although the TP receptor appears to be upregulated at gestation and post-partum. The results also show that myometrial prostanoid release in the human uterus is cyclically regulated, with the greatest amount of prostaglandins being released during the late follicular stage. In conclusion, although similarities do exist with regard to the ovarian regulation of uterine motility in both the rat and human uterus, the differences in the apparent functional prostaglandin receptor populations between the two species suggest further work is required before the rat can be used as a model of human uterine function.en_US
dc.description.sponsorshipAllergan Incen_US
dc.language.isoenen_US
dc.rights<a rel="license" href="http://creativecommons.org/licenses/by-nc-nd/3.0/"><img alt="Creative Commons License" style="border-width:0" src="http://i.creativecommons.org/l/by-nc-nd/3.0/88x31.png" /></a><br />The University of Bradford theses are licenced under a <a rel="license" href="http://creativecommons.org/licenses/by-nc-nd/3.0/">Creative Commons Licence</a>.eng
dc.subjectProstaglandinsen_US
dc.subjectMyometriumen_US
dc.subjectHumanen_US
dc.subjectRaten_US
dc.subjectMenstrual cycleen_US
dc.subjectOestrous cycleen_US
dc.subjectPregnancyen_US
dc.subjectProstanoid receptorsen_US
dc.titleThe effect of prostaglandins in myometrial tissue; a functional and lipidomic study. The influence of the hormonal milieu on the functional response to prostaglandins and ex vivo lipid biosynthesis in myometrial tissues.en_US
dc.type.qualificationleveldoctoralen_US
dc.publisher.institutionUniversity of Bradfordeng
dc.publisher.departmentBradford School of Pharmacyen_US
dc.typeThesiseng
dc.type.qualificationnamePhDen_US
dc.date.awarded2012
refterms.dateFOA2018-07-19T12:18:32Z


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