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dc.contributor.advisorRandall, Valerie A.
dc.contributor.authorAl-Waleedi, Saeed A.*
dc.date.accessioned2011-12-07T14:04:35Z
dc.date.available2011-12-07T14:04:35Z
dc.date.issued2010
dc.identifier.urihttp://hdl.handle.net/10454/5271
dc.description.abstractAndrogens are the main regulators of human hair growth stimulating larger, terminal hair development e.g. beard and causing scalp balding, androgenetic alopecia. Hair disorders cause psychological distress but are poorly controlled. Androgens probably act by altering regulatory paracrine factors produced by the mesenchyme-derived dermal papilla. This study aimed to investigate paracrine factors involved in androgen-regulated alopecia, particularly hepatocyte growth factor (HGF) family members, by investigating their in vivo status. Balding and non-balding scalp hair follicles and their component tissues were isolated and analysed by molecular biological methods (reverse transcriptase-polymerase chain reaction (RT-PCR), quantitative PCR and DNA microarray analysis), cell culture and immunohistochemistry. Scalp follicles expressed a range of paracrine messenger genes. The dermal papilla, cultured dermal papilla cells and dermal sheath expressed several HGF family genes, while matrix cells only produced the receptor RON suggesting autocrine roles for HGF and MSP, but a paracrine route only for MSP. Comparing balding and non-balding follicles from the same individuals revealed the expected reduction in several keratin and keratin-related protein genes supporting this approach's validity. There were also significant differences in paracrine factors previously implicated in androgen action by in vitro studies. Several factors believed to increase during androgen stimulation of larger, darker follicles, e.g. IGF-I and SCF, were lowered in balding follicles, while putative inhibitory factors, e.g. TGFß-1, were increased. HGF and MSP and their receptors, c-Met and RON, were significantly reduced. These results increase our understanding of androgen action in human hair follicles; this could lead to better treatments for hair disorders.en_US
dc.description.sponsorshipSaudi governmenten_US
dc.language.isoenen_US
dc.rights© 2010 Al-Waleedi, S. A. This work is licensed under a Creative Commons Attribution-Non-Commercial-Share-Alike License (http://creativecommons.org/licenses/by-nc-nd/2.0/uk).en_US
dc.subjectHGFen_US
dc.subjectMSPen_US
dc.subjectc-Meten_US
dc.subjectRONen_US
dc.subjectHair folliclesen_US
dc.subjectAndrogenen_US
dc.subjectAndrogenetic alopeciaen_US
dc.subjectPCRen_US
dc.subjectGene microarrayen_US
dc.subjectBaldingen_US
dc.subjectImmunohistochemistryen_US
dc.titleThe roles of hepatocyte growth factor family members in androgen-regulation of human hair growth. A comparison of the expression of hepatocyte growth factor family members, HGF and MSP, and their receptors, c-Met and RON, in isolated hair follicles from normal and androgenetic alopecia (balding) scalp.en_US
dc.type.qualificationleveldoctoralen_US
dc.publisher.institutionUniversity of Bradfordeng
dc.publisher.departmentDepartment of Biomedical Sciencesen_US
dc.typeThesiseng
dc.type.qualificationnamePhDen_US
dc.date.awarded2010
refterms.dateFOA2017-07-05T00:00:00Z


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