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dc.contributor.authorRhodes, L.E.*
dc.contributor.authorGledhill, Karl*
dc.contributor.authorMasoodi, Mojgan*
dc.contributor.authorHaylett, A.K.*
dc.contributor.authorBrownrigg, M.*
dc.contributor.authorThody, Anthony J.*
dc.contributor.authorTobin, Desmond J.*
dc.contributor.authorNicolaou, Anna*
dc.date.accessioned2010-12-16T11:55:37Z
dc.date.available2010-12-16T11:55:37Z
dc.date.issued2009
dc.identifier.citationRhodes, L. E., Gledhill, K., Masoodi, M., Haylett, A. K., Brownrigg, M., Thody, A. J., Tobin, D. J. and Nicolaou, A. (2009). The sunburn response in human skin is characterized by sequential eicosanoid profiles that may mediate its early and late phases. FASEB Journal, Vol. 23, No.11, pp. 3947¿3956.en
dc.identifier.urihttp://hdl.handle.net/10454/4586
dc.descriptionyesen
dc.description.abstractSunburn is a commonly occurring acute inflammatory process, with dermal vasodilatation and leukocyte infiltration as central features. Ultraviolet (UV) B-induced hydrolysis of membrane phospholipids releases polyunsaturated fatty acids and their subsequent metabolism by cyclooxygenases (COX) and lipoxygenases (LOX) may produce potent eicosanoid mediators modulating different stages of the inflammation. Our objective was to identify candidate eicosanoids formed during the sunburn reaction in relation to its clinical and histological course. We exposed skin of healthy humans (n=32) to UVB and for 72h examined (i) expression of pro- and anti-inflammatory eicosanoids using LC/ESI-MS/MS and (ii) immunohistochemical expression of COX-2, 12-LOX, 15-LOX and leucocyte markers, while (iii) quantifying clinical erythema. We show that vasodilatory prostaglandins (PG)E2, PGF2¿ and PGE3 accompany the erythema in the first 24-48h, associated with increased COX-2 expression at 24h. Novel, potent leukocyte chemoattractants 11-, 12- and 8-monohydroxy-eicosatetraenoic acid (-HETE) are elevated from 4-72h, in association with peak dermal neutrophil influx at 24h, and increased dermal CD3+ lymphocytes and 12- and 15-LOX expression from 24-72h. Anti-inflammatory metabolite 15-HETE shows later expression, peaking at 72h. Sunburn is characterized by overlapping phases of increases in COX products followed by LOX products that may regulate subsequent events and ultimately its resolution.en
dc.description.sponsorshipThe Wellcome Trusten
dc.language.isoenen
dc.subjectHydroxyeicostetraenoic acidsen
dc.subjectProstaglandinsen
dc.subjectTandem mass spectrometryen
dc.subjectCyclooxygenaseen
dc.subjectLipoxygenaseen
dc.subjectLeucocytesen
dc.subjectSunburn responseen
dc.titleThe sunburn response in human skin is characterized by sequential eicosanoid profiles that may mediate its early and late phases.en
dc.status.refereedyesen
dc.typeArticleen
dc.type.versionpublished version paperen
dc.identifier.doihttps://doi.org/10.1096/fj.09-136077
refterms.dateFOA2018-07-19T04:23:25Z


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